Fig. 5

Fractionation of the surface displaying bacterial cells; a lane 1: outer membrane fraction of the cell surface from recombinant E. coli without sortase treatment. The band corresponding to the whole chimeric protein is indicated with arrow. a Lane 2: outer membrane fraction of the cell surface from E. coli after treatment with sortase. The density of the band related to the whole chimeric protein is decreased. b Lane 1: outer membrane fraction of the cell surface from E. coli after treatment with sortase, Lpp-ompA remains in outer membrane fraction (a band of 14 kDa indicated by arrow). Lane 2: outer membrane fraction of the cell surface from E. coli without treatment with sortase