Poly-l-gamma-glutamic acid production by recombinant Bacillus subtilis without pgsA gene

AMB Express

Table 3 Chirality of PGA from transformants

Strain	Medium^a	OD₆₀₀	Mw/10³	Prod (g/L)	d/l-ratio
P_BC/Δ3	l-Glu	29.6	2000	35.3	4/96
P_BC/Δ3	d-Glu	17.5	600	3.1	4/96
P_Bam-BC/Δ3	l-Glu	37.9	1100	2.5	4/96
P_Bli-BC/Δ3	l-Glu	36.4	2800	1.4	2/98
P_Obi-BC/Δ3	l-Glu	39.0	1900	0.7^b	11/89

^aMedium; l-Glu was used 2xL/Mal+E8 (which contained 0.43M l-glutamic acid) and d-Glu was used 2xL/Mal, containing 0.43M d-glutamic acid and adjusted to the same pH as 2xL/Mal+E8 with NaOH. Data are presented as means of five independent experiments
^bPGA production of P_Obi-BC/Δ3 strains is representative of three-fifth or two-fifth of positive transformants (it seems to be unstable)