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Table 2 Growth and PGA production by Bacillus transformants

From: Poly-l-gamma-glutamic acid production by recombinant Bacillus subtilis without pgsA gene

Strain

2xL/Mal

2xL/Mal+E8

OD600

Mw/103

Prod (g/L)

OD600

Mw/103

Prod (g/L)

P_300/3

34 ± 3.3

nd

nd

47 ± 1.6

nd

nd

P_BCA/3

34 ± 6.2

nd

nd

40 ± 10

1000 ± 200

3.0 ± 0.7

P_BC/3

14 ± 4.1

400 ± 100

4.0 ± 0.3

16 ± 4.6

1100 ± 200

26.0 ± 3.1

P_BA/3

38 ± 2.7

nd

nd

30 ± 2.8

nd

nd

P_CA/3

38 ± 1.0

nd

nd

41 ± 3.2

nd

nd

P_B/3

33 ± 3.8

nd

nd

27 ± 2.4

nd

nd

P_C/3

34 ± 1.6

nd

nd

40 ± 2.3

nd

nd

P_A/3

37 ± 1.5

nd

nd

47 ± 2.3

nd

nd

  1. Data are presented as means of five independent experiments ± SE (n = 5)