Table 1 Primers used for construction of mutants and plasmids
From: Poly-l-gamma-glutamic acid production by recombinant Bacillus subtilis without pgsA gene
Primer names | Sequence (5′→3′) |
|---|---|
Cmr Fw | CAACTAAAGCACCCATTAGTTCAACAAACG |
Cmr Rv | CTTCAACTAACGGGGCAGGTTAGTGAC |
BCA-Dw Fw | CAAGCCCCGAGCAATCA |
BCA-Dw Rv | CTAATGGGTGCTTTAGTTGTCGGAGTGATAAAGATGAAATTTGTC |
BCA-Up Fw | CTGCCCCGTTAGTTGAAGTGCTTTTCGACATCTCCTT |
BCA-Up Rv | AAGGGTTTGTGATATCCGG |
P_S237 Fw | CAACTAAAGCACCCATTAGGGATCCAACAGGCTTATATTTAGAG |
P_S237 Rv | CATCATATTACCTCCTAAATATTTTTAAAGTA |
Bsu pgsA Rv | CGAAGCTTAGATGGCTTTGACAAATTTCATC |
Bsu pgsC Rv | CCCAAGCTTGACCTTCGGCGTTTCCGCT |
Bsu pgsB Rv | CCCAAGCTTGGCAGCGAATTTTCTGCGTCC |
Bsu pgsA Fw | ATTTAGGAGGTAATATGATGAAAAAAGAACTGAGCTTTCATG |
Bsu pgsC Fw | ATTTAGGAGGTAATATGATGTTCGGATCAGATTTATACATC |
Bsu pgsB Fw | ATTTAGGAGGTAATATGATGTGGTTACTCATTATAGCCTG |
Bsu pgsBA Fw | CGTAAGCTAGGGGGAAATGCAGACGATGAAAAAAGAACTGAGCTTTCATG |
pHY-P_S237 Rv | CATCATATTACCTCCTAAATATTTTTAAAGTA |
pHY Fw | TAGAAGCTTGGGCAAAGCGTTTTTCCA |
Bam pgsB Fw | GGAGGTAATATGATGTGGTTACTCATTATAGCCTGTG |
Bli pgsB Fw | GGAGGTAATATGATGTGGGTAATGCTATTAGCCTGTG |
Obi pgsB Fw | GGAGGTAATATGATGCTAGAAGAATTTTTAATCATTT |
Bam pgsC Rv | TTGCCCAAGCTTCTACCTTCCCTGTCAGGATGCAG |
Bli pgsC Rv | TTGCCCAAGCTTCTACTTCCGACCCAAGTAAAGAC |