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Fig. 1 | AMB Express

Fig. 1

From: Poly-l-gamma-glutamic acid production by recombinant Bacillus subtilis without pgsA gene

Fig. 1

Construction of pgsBCA deletion mutants and pgs-gene expression vectors. a Deletion of pgsBCA genes from B. subtilis Marburg No.168 by double crossover homologous recombination. b Construction of pgs-gene expression vectors. DNA fragments (pgsBCApgsA) were amplified using spliced overlapping-extension (SOE)-PCR and were cloned into the plasmid pHY300PLK using restriction enzyme digestion. The heterologous Bacillus pgsBC fragments Bam-pgsBC, Bli-pgsBC, and Obi-pgsBC were then cloned using an in-fusion recombination cloning system

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