Fig. 1From: Poly-l-gamma-glutamic acid production by recombinant Bacillus subtilis without pgsA geneConstruction of pgsBCA deletion mutants and pgs-gene expression vectors. a Deletion of pgsBCA genes from B. subtilis Marburg No.168 by double crossover homologous recombination. b Construction of pgs-gene expression vectors. DNA fragments (pgsBCA–pgsA) were amplified using spliced overlapping-extension (SOE)-PCR and were cloned into the plasmid pHY300PLK using restriction enzyme digestion. The heterologous Bacillus pgsBC fragments Bam-pgsBC, Bli-pgsBC, and Obi-pgsBC were then cloned using an in-fusion recombination cloning systemBack to article page