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Table 1 The confirmative results on spore detection by DPA fluorimetry assay of B. subtilis MA139 and B. licheniformis BL20386 compared with Plate counting assay

From: Rapid and simple detection of endospore counts in probiotic Bacillus cultures using dipicolinic acid (DPA) as a marker

Sampling time (h)

B. subtilis MA139 concentration (CFU/mL)

B. licheniformis BL20386 concentration (CFU/mL)

DPA fluorimetry assay

Plate counting assay

DPA fluorimetry assay

Plate counting assay

12

(4.35 ± 0.21) × 104

(4.05 ± 0.35) × 104

(5.85 ± 0.63) × 104

(5.55 ± 0.78) × 104

24

(5.65 ± 0.21) × 105

(5.50 ± 0.36) × 105

(8.30 ± 0.28) × 105

(7.90 ± 1.27) × 105

36

(4.32 ± 0.11) × 107

(3.80 ± 0.35) × 107

(4.70 ± 0.14) × 107

(4.30 ± 1.27) × 107