Development of a species-specific TaqMan-MGB real-time PCR assay to quantify Olsenella scatoligenes in pigs offered a chicory root-based diet

AMB Express

Table 2 Reproducibility, intra-assay, and inter-assay coefficient of variation for TaqMan qPCR

No. of SK9K4^T genome (copies/reaction)	Intra-assay CT value	CV (%)	Inter-assay CT value	CV (%)
3 × 10⁶	13.30 ± 0.20	1.49	13.26 ± 0.10	0.74
3 × 10⁵	16.94 ± 0.07	0.42	16.86 ± 0.13	0.80
3 × 10⁴	20.33 ± 0.04	0.19	20.01 ± 0.48	2.40
3 × 10³	23.73 ± 0.09	0.37	23.62 ± 0.13	0.56
3 × 10²	27.17 ± 0.23	0.85	27.80 ± 0.12	0.58
3 × 10¹	30.46 ± 0.32	1.05	30.38 ± 0.22	0.72
3 × 10⁰	33.93 ± 0.46	0.83	33.73 ± 0.25	0.73