Analysis and expression of the carotenoid biosynthesis genes from Deinococcus wulumuqiensis R12 in engineered Escherichia coli

AMB Express

Table 2 Primers used in this work

Genes	Primer sequence^a	Restriction enzyme site
crtE1	F: 5′- GATCCATATGCGTCCCGAACTG -3′	NdeI
crtE1	R: 5′- CTTGAATTCCTTCTCCCGCGTCGC -3′	EcoRI
crtB1	F: 5′- CCGGAATTCGTGACGGAATTTTCGCC -3′	EcoRI
crtB1	R: 5′- CCCAAGCTTGCCGTGGGCGGCGTC -3′	HindIII
crtI1	F: 5′-CCCAAGCTTATGACATCCCCTCTTCCCTG -3′	HindIII
crtI1	R: 5′- CCGCTCGAGTCAGCGCCGGATGTCG -3′	XhoI
crtI2	F: 5′- CCGGAATTCATGACATCCCCTCTTCCCTG -3′	EcoRI
crtI2	R: 5′- CCCAAGCTTGCGCCGGATGTCG -3′	HindIII
crtB2	F: 5′-CCCAAGCTTGTGACGGAATTTTCGCC -3′	HindIII
crtB2	R: 5′- CCGCTCGAGTCAGCCGTGGGCGGCGTC -3′	XhoI
crtB3	F: 5′- GATCCATATGGTGACGGAATTTTCGCC -3′	NdeI
crtB3	R: 5′- CTTGAATTCGCCGTGGGCGGCGTC -3′	EcoRI
crtE2	F: 5′- CCGGAATTCATGCGTCCCGAACTG -3′	EcoRI
crtE2	R: 5′- CCCAAGCTTCTTCTCCCGCGTCGC -3′	HindIII
crtE3	F: 5′- CCCAAGCTTATGCGTCCCGAACTG -3′	HindIII
crtE3	R: 5′- CCGCTCGAGTCACTTCTCCCGCGTCGC -3′	XhoI
crtI3	F: 5′- GATCCATATGATGACATCCCCTCTTCCCTG -3′	NdeI
crtI3	R: 5′- CTTGAATTCGCGCCGGATGTCG -3′	EcoRI