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Table 1 Summary of two-column-step IgG purification process

From: Application of enhanced electronegative multimodal chromatography as the primary capture step for immunoglobulin G purification

 

CCS

c-e CCS

c-e CCS > enhanced HCX

c-e CCS > enhanced HCX > VEAX

IgG (mg/mL)

1.45

1.12

15.9

10.6

Stepwise IgG recovery (%)

100

91.8

94.6

99.3

DNA (ppm)

10,600

0.05

0.006

0.0004

Histone HCP (ppm)

28,560

< LOD

< LOD

< LOD

nh-HCP (ppm)

224,800

8380

296

0.6

Aggregates (%)

18.65

0.45

0.42

0.40

LC (%)

12.3

1.2

< 0.05

< 0.05

Turbidity (NTU)

25.6

3.11

2.15

1.88

Caprylic acid (μg/mL)

NA

5.67

2.32

0.14

Allantoin (μg/mL)

NA

1119.05

< LOD

< LOD

  1. NA not applicable