Skip to main content

Table 1 The kinetic values of purified recombinant TlLipA towards esters of different lengths

From: An alkaline and surfactant-tolerant lipase from Trichoderma lentiforme ACCC30425 with high application potential in the detergent industry

Substrate

V max (μmol/min mg)

k cat (/s)

K m (mM)

k cat /K m (/s mM)

pNPO (C8)

27.3 ± 4.4

27.4 ± 4.4

0.67 ± 0.23

41.0

pNPD (C10)

12.5 ± 0.7

12.5 ± 0.7

0.35 ± 0.07

35.8

pNPB (C4)

12.2 ± 0.8

12.2 ± 0.8

0.40 ± 0.07

30.7

pNPDD (C12)

16.8 ± 2.4

16.8 ± 2.4

1.59 ± 0.42

10.6

pNPM (C14)

4.3 ± 1.0

4.3 ± 1.0

3.29 ± 1.21

1.32

pNPP (C16)

4.8 ± 1.0

4.8 ± 1.0

4.22 ± 1.28

1.14