Skip to main content

Table 1 Primers used in this study

From: A study on the use of strain-specific and homologous promoters for heterologous expression in industrial Saccharomyces cerevisiae strains

Primers Fragment (approximate length) Restriction endonuclease Sequence (5′→3′)a
pPGKSACI-F PGK1 (1500 bp) SacI ttcGAGCTCtctaactgatctatccaaaactga
pPGKBAM-R BamHI tGGATCCtgttttatatttgttgtaaaaagtaga
ADHSAC-F ADH1 (1500 bp) SacI aGAGCTCattaaaacaagaagagggttgac
ADHBAM-R BamHI aGGATCCtgtatatgagatagttgattgtatg
PGISACI-F PGI1 (400 bp) SacI aGAGCTCgtgggtgtattggattatagg
PGIBAM-R BamHI aGGATCCtttttaggctggatcttgattcta
CYCpp5 CYC1 (300 bp) SacI tGAGCTCgagctcatttggcgagcgtt
CYCpp3 BamHI tGGATCCgattagtgtgtgtatttgtgtttg
TEFP5 TEF1 (400 bp) SacI gGAGCTCccccacacaccatagcttcaa
TEFP3 BamHI cGGATCCtttgtaattaaaacttagattagattg
eGFPBAM-F eGFP (770 bp) BamHI aGGATCCatggtgagcaagggcgagga
eGFPNOT-R NotI gGCGGCCGCttacttgtagagctc
GPD5 ZWF1 (1500 bp) BamHI cGAATTCaagatgagtgaaggccccgt
GPD3 BamHI cGTCGACattatccttcgtatcttctggc
  1. aRestriction site are in capital letters