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Table 1 Primers used in this study

From: A study on the use of strain-specific and homologous promoters for heterologous expression in industrial Saccharomyces cerevisiae strains

Primers

Fragment (approximate length)

Restriction endonuclease

Sequence (5′→3′)a

pPGKSACI-F

PGK1 (1500 bp)

SacI

ttcGAGCTCtctaactgatctatccaaaactga

pPGKBAM-R

BamHI

tGGATCCtgttttatatttgttgtaaaaagtaga

ADHSAC-F

ADH1 (1500 bp)

SacI

aGAGCTCattaaaacaagaagagggttgac

ADHBAM-R

BamHI

aGGATCCtgtatatgagatagttgattgtatg

PGISACI-F

PGI1 (400 bp)

SacI

aGAGCTCgtgggtgtattggattatagg

PGIBAM-R

BamHI

aGGATCCtttttaggctggatcttgattcta

CYCpp5

CYC1 (300 bp)

SacI

tGAGCTCgagctcatttggcgagcgtt

CYCpp3

BamHI

tGGATCCgattagtgtgtgtatttgtgtttg

TEFP5

TEF1 (400 bp)

SacI

gGAGCTCccccacacaccatagcttcaa

TEFP3

BamHI

cGGATCCtttgtaattaaaacttagattagattg

eGFPBAM-F

eGFP (770 bp)

BamHI

aGGATCCatggtgagcaagggcgagga

eGFPNOT-R

NotI

gGCGGCCGCttacttgtagagctc

GPD5

ZWF1 (1500 bp)

BamHI

cGAATTCaagatgagtgaaggccccgt

GPD3

BamHI

cGTCGACattatccttcgtatcttctggc

  1. aRestriction site are in capital letters