Primers | Fragment (approximate length) | Restriction endonuclease | Sequence (5′→3′)a |
---|---|---|---|
pPGKSACI-F | PGK1 (1500Â bp) | SacI | ttcGAGCTCtctaactgatctatccaaaactga |
pPGKBAM-R | BamHI | tGGATCCtgttttatatttgttgtaaaaagtaga | |
ADHSAC-F | ADH1 (1500Â bp) | SacI | aGAGCTCattaaaacaagaagagggttgac |
ADHBAM-R | BamHI | aGGATCCtgtatatgagatagttgattgtatg | |
PGISACI-F | PGI1 (400Â bp) | SacI | aGAGCTCgtgggtgtattggattatagg |
PGIBAM-R | BamHI | aGGATCCtttttaggctggatcttgattcta | |
CYCpp5 | CYC1 (300Â bp) | SacI | tGAGCTCgagctcatttggcgagcgtt |
CYCpp3 | BamHI | tGGATCCgattagtgtgtgtatttgtgtttg | |
TEFP5 | TEF1 (400Â bp) | SacI | gGAGCTCccccacacaccatagcttcaa |
TEFP3 | BamHI | cGGATCCtttgtaattaaaacttagattagattg | |
eGFPBAM-F | eGFP (770Â bp) | BamHI | aGGATCCatggtgagcaagggcgagga |
eGFPNOT-R | NotI | gGCGGCCGCttacttgtagagctc | |
GPD5 | ZWF1 (1500Â bp) | BamHI | cGAATTCaagatgagtgaaggccccgt |
GPD3 | BamHI | cGTCGACattatccttcgtatcttctggc |