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Fig. 2 | AMB Express

Fig. 2

From: A study on the use of strain-specific and homologous promoters for heterologous expression in industrial Saccharomyces cerevisiae strains

Fig. 2

Transcription analysis of eGFP gene under control of P ADH1 and P PGK1 from JPU and S288C in S. cerevisiae JPU. Total yeast RNA was prepared, run in a 1.5% agarose/formaldehyde gel and transferred to a nitrocellulose membrane. The membrane was probed with the eGFP PCR product (a) and with the ZWF1 PCR product as loading control (b). Lanes: 1 non-transformed cell; 2 Y2JADH; 3 Y2SADH; 4 Y2JPGK; 5 Y2SPGK. The predicted size of eGFP mRNA is approximately 0.7 kb and SWF1 mRNA is ~ 1.5 kb

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