Rapid monitoring of the target protein expression with a fluorescent signal based on a dicistronic construct in Escherichia coli

AMB Express

Table 2 Primers used in this study

Primer	Sequence 5′–3′	Restriction site
1mF	GGATCTGCATATGGTCTCTAAAGGCGAGGAAG	NdeI
1mR	CGGGATCCTTATTTGTACAGTTCGTCCATG	BamHI
2mF	CCCAAGCTTAAGAAGGAGATATACATATG GTCTCTAAAGGCG	HindIII
2mR	CCGCTCGAGTTATTTGTACAGTTCGTCCATG	XhoI
2m1F	GGAATTCCTAGAGCTCCGTCGACAAG	EcoRI
2m2F	GGAATTCCTAATAGAGCTCCGTCGACAAG	EcoRI
2m3F	GGAATTCCTAATAAGGGAGCTCCGTCGACAAG	EcoRI
2eF	CGGGATCCATGGTAAGCAAGGGTGAG	BamHI
2eR	CCCAAGCTTCTTTGTACAGTTCATCCATG	HindIII
2e1F	CGGGATCCATGCTAGTAAGCAAGGGTGAG	BamHI
2e2F	CGGGATCCATGCTAATAGTAAGCAAGGGTGAG	BamHI

Rare codons are represented in bold, and restriction enzyme sites are indicated with underline