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Table 1 Bacterial strains and plasmids used in this study

From: Rapid monitoring of the target protein expression with a fluorescent signal based on a dicistronic construct in Escherichia coli

  Genotype/characteristics Source
Strains
 Top10 F mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG Invitrogen
 BL21(DE3)pLysS F ompT hsdS B (r B m B ) gal dcm (DE3) pLysS (CmR) Novagen
 Rosetta(DE3)pLysS F ompT hsdS B (r B m B ) gal dcm lacY1 (DE3) pLysSRARE (CmR) Novagen
Plasmids
 pET-21a Ampr T7 promoter lac operator Novagen
 pUCm-T TA cloning Sangon
 pT-RFP pUCm-T carrying mcherry This study
 pET-RFP pET-21a expressing mCherry This study
 pO-RFP pET-21a carrying mcherry in ORF2 This study
 pOI-RFP pO-RFP with one rare codon in ORF1 This study
 pOII-RFP pO-RFP with two consecutive rare codons in ORF1 This study
 pOIII-RFP pO-RFP with three consecutive rare codons in ORF1 This study
 pT-GFP pUCm-T carrying egfp This study
 pGFP-RFP pO-RFP carrying egfp in ORF1 This study
 pGFPI-RFP pGFP-RFP with one rare codon preceding egfp This study
 pGFPII-RFP pGFP-RFP with two consecutive rare codons preceding egfp This study