Table 1 Bacterial strains and plasmids used in this study
Genotype/characteristics | Source | |
|---|---|---|
Strains | ||
Top10 | F− mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG | Invitrogen |
BL21(DE3)pLysS | F− ompT hsdS B (r − B m − B ) gal dcm (DE3) pLysS (CmR) | Novagen |
Rosetta(DE3)pLysS | F− ompT hsdS B (r − B m − B ) gal dcm lacY1 (DE3) pLysSRARE (CmR) | Novagen |
Plasmids | ||
pET-21a | Ampr T7 promoter lac operator | Novagen |
pUCm-T | TA cloning | Sangon |
pT-RFP | pUCm-T carrying mcherry | This study |
pET-RFP | pET-21a expressing mCherry | This study |
pO-RFP | pET-21a carrying mcherry in ORF2 | This study |
pOI-RFP | pO-RFP with one rare codon in ORF1 | This study |
pOII-RFP | pO-RFP with two consecutive rare codons in ORF1 | This study |
pOIII-RFP | pO-RFP with three consecutive rare codons in ORF1 | This study |
pT-GFP | pUCm-T carrying egfp | This study |
pGFP-RFP | pO-RFP carrying egfp in ORF1 | This study |
pGFPI-RFP | pGFP-RFP with one rare codon preceding egfp | This study |
pGFPII-RFP | pGFP-RFP with two consecutive rare codons preceding egfp | This study |