Table 1 Purification steps of neutral protease from Aspergillus oryzae Y1
Purification steps | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Purification (fold) | Recovery (%) |
|---|---|---|---|---|---|
Crude enzyme solution | 439,062 | 1934.7 | 226.9 | 1.0 | 100 |
(NH4)2SO4 (70%) | 316,124 | 383.9 | 823.4 | 3.6 | 72.0 |
DEAEsepharose-FF | 92,389 | 40.8 | 2264.3 | 10.0 | 21.0 |