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Table 1 Relative quantification and exression multiplicity of nanH, cpp, pld, sodC and spaC genes from Corynebacterium pseudotuberculosis

From: In vivo and in vitro expression of five genes involved in Corynebacterium pseudotuberculosis virulence

Sample

mRNA

Quantification fator in the abscess

Quantification fator in the culture

Normalized Quantification fator in the abscess × 1000

Normalized Quantification fator in the culture × 1000

Expression multiplicity

Animal 1

rna16S

12,213

1,693,182

   
 

nanH

10,245

516,504

838,860

305,049

2750

 

Cpp

7786

449,959

637,517

265,748

2399

 

Pld

7203

754,682

589,781

445,718

1323

 

sodC

12,990

710,035

1,063,621

419,349

2536

 

spaC

11,330

611,099

927,700

360,917

2570

Animal 2

rna16S

10,751

3,587,966

   
 

nanH

14,466

843,655

1,345,549

235,135

5722

 

Cpp

11,368

756,508

1,057,390

210,846

5015

 

Pld

11,753

939,822

1,093,201

261,937

4174

 

sodC

17,348

1,055,413

1,613,617

294,154

5486

 

spaC

14,273

1,025,113

1,327,597

285,709

4647

Animal 3

rna16S

12,109

2,490,341

   
 

nanH

17,684

512,642

1,460,401

205,852

7094

 

Cpp

13,546

429,309

1,118,672

172,390

6489

 

Pld

15,912

460,927

1,314,064

185,086

7100

 

sodC

20,697

577,854

1,709,225

232,038

7366

 

spaC

20,171

601,448

1,665,786

241,512

6897

Animal 4

rna16S

24,930

4,981,860

   
 

nanH

8376

161,958

335,981

32,510

10,335

 

Cpp

7063

129,867

283,313

26,068

10,868

 

Pld

7327

213,745

293,903

42,905

6850

 

sodC

9982

215,407

400,401

43,238

9260

 

spaC

8903

189,375

357,120

38,013

9395

Animal 5

rna16S

13,377

2,012,331

   
 

nanH

18,657

187,995

1,394,707

93,422

14,929

 

Cpp

15,847

196,526

1,184,645

97,661

12,130

 

Pld

17,692

254,409

1,322,569

126,425

10,461

 

sodC

22,484

260,336

1,680,795

129,370

12,992

 

spaC

20,163

292,056

1,507,289

145,133

10,386