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AMB Express

Table 2 Primers used in this study

From: Ribosomal binding site sequences and promoters for expressing glutamate decarboxylase and producing γ-aminobutyrate in Corynebacterium glutamicum

Primers Sequences (5′–3′) Restriction sites
R-B2-F GGGGTTAACAGAAGGAGGGATTGCATGAATAAAAACGATCAGGA HpaI
gadB2-R CGGGATCCTTAACTTCGAACGGTGGTCT BamHI
R1-B2-F CGGGGCTTAAGAAAAGGAGGGATTGCATGAATAAAAACGATCAGGA AflII
R2-B2-F CGGGCTTAAGGGAAGGAGGGATTGCATGAATAAAAACGATCAGGA AflII
R3-B2-F GCCCTTAAGGAAAGGCGAGGATTGCATGAATAAAAACGATCAGGA AflII
R4-B2-F CGGGGCTTAAGGAAAGGAGAGGATTGCATGAATAAAAACGATCAGGA AflII
R5-B2-F CGGGCTTAAGGAAAGGAGGGATTGCATGAATAAAAACGATCAGGA AflII
R6-B2-F CGGGCTTAAGAGGAGGAGGGATTGCATGAATAAAAACGATCAGGA AflII
R7-B2-F CGGGGCTTAAGGAAAGGAGGATTGCATGAATAAAAACGATCAGGA AflII
R8-B2-F CGGGGCTTAAGAGAAAGGAGGGGATTGCATGAATAAAAACGATCAGGA AflII
R9-B2-F CGGGGCTTAAGGAAAGGAGGGGATTGCATGAATAAAAACGATCAGGA AflII
R10-B2-F GGCGCTTAAGGAGAGGAGGGATTGCATGAATAAAAACGATCAGGA AflII
R11-B2-F CGGGGCTTAAGAAGAGGAGGGATTGCATGAATAAAAACGATCAGGA AflII
R12-B2-F CGGGGCTTAAGGGGAGGAGGGATTGCATGAATAAAAACGATCAGGA AflII
R4a-B2-F GCCGGCTTAAGGAAAGGAGAGGATTGATGAATAAAAACGATCAGGA AflII
R4b-B2-F GCCGGCTTAAGGAAAGGAGAGGATTGCAATGAATAAAAACGATCAGGA AflII
R4a-B1mut-F GGAAGATCTGAAAGGAGAGGATTGATGGCTATGTTGTATGGAA BglII
gadB1mut-R AACTGCAGTTAGTGCGTGAACCCGTATTTTTT PstI
M-R4a-B1mut-F ACGCGTCGACGGTTCTGGCAAATATTCTGAAA SalI
P sod -F CTAGTCTAGACTTATGCCCTTCAACCCTACTTA XbaI
P sod -R GCAAGATCTAGATTCGTAGGTTTCCGCACCGAGCATA XbaI
P uspA -F CTAGTCTAGACGAGATGAGATCTTCGAGCT XbaI
P uspA -R GCGGACTCTAGAAAACTTATGACAGGGGTTAAAA XbaI
P cspB -F CTAGTCTAGATAGTCAAGAATTTACCCCCT XbaI
P cspB -R CTAGTCTAGATTGAACACTGCGCACTGAAA XbaI
P tuf -F CATTTCTAGATAAGTGGGGTAGCGGCTTGT XbaI
P tuf -R CCGCTATCTAGAAATTGGTTTTGCTTTCACTG XbaI
P gdh -F CCTAGTCTAGAGAAGAGACTTCATGCAGTTACC XbaI
P gdh -R CCGGTCTAGAACGATTTTAAAGTGTGTATCTG XbaI
P dtsR -F CTAGTCTAGACACGCCCAAAAAGTTTTACC XbaI
P dtsR -R CCTAGTCTAGATGTTTTGAAATCGTAGCGGTA XbaI
P odhI -F CATTTCTAGACGATCACGAGGGGGCACATT XbaI
P odhI -R CCCGGCTCTAGATCTTAACGATTTTCATCATA XbaI
P sigB -F ATATTCTAGATTAACGAAGGCCCCTTCACC XbaI
P sigB -R GTATTCTAGAGGTTCAAGAGGTTCAACGGA XbaI
P hmp -F CTCCTCTAGACGATAAAACGGCATTAAACC XbaI
P hmp -R CGGGTCTAGAGCTAATTTCTACGGATTTAATC XbaI
P pqo -F GTCCTCTAGAAGCAACGACGGAAATCCCAAAA XbaI
P pqo -R GTTCTCTAGATGCCTAACTTGGTGCGACTT XbaI
P gapA -F GCCATCTAGAAAACTATTTAGCGCAAGTGT XbaI
P gapA -R GCCATCTAGATGTAGGAAATGCAATGTGTC XbaI
P ilvE -F ATACTCTAGAGGGGGAGGCATCAAATTGA XbaI
P ilvE -R GCGCTCTAGATCATGGATTTTAAGGTACAC XbaI
P cg1417 -F GTCCTCTAGAGTGTCGTTTTTTGTGCACCTGTTTTG XbaI
P cg1417 -R GGGGTCTAGAGAAAATCCAGGTTAAAAGC XbaI
P dnaK -F CGCGTCTAGAATTGGGTGGTTGAAAATTAG XbaI
P dnaK -R CGCGTCTAGAGTGATTTTAGTACTGTCCA XbaI
P clgR -F ATATTCTAGAAACAAGATGGTCATCCGGTG XbaI
P clgR -R GCTTTCTAGACGTTAGGTTCAACTCCCTTT XbaI
P clpB -F ATATTCTAGATGATTTTTGGCCTCGCGTGG XbaI
P clpB -R CGATTCTAGAGCCAACCTACACAATCAGT XbaI
P dnaJ -F CATTTCTAGATTAGTGGTTTCCGCCGTTGT XbaI
P dnaJ -R CAGGTCTAGAGAGTCTTATATGCGGTGAAT XbaI
P sufR -F CTAGTCTAGATACCTTTGGTTGGCTTAGGG XbaI
P sufR -R CTAGTCTAGATGGTGTCACCTCCTGCTTGA XbaI
P trxB1 -F CTAGTCTAGACCGCAACAATGCCGATTTCA XbaI
P trxB1 -R CGCGTCTAGATTCAAGATTTGTAAGGTCTA XbaI
P dnaK -2SD-R CATTAATCCTCTCCTTTCGAGTTGGTGGTTCCAAGGTCA  
P dnaK -2SDB2-F CCACCAACTCGAAAGGAGAGGATTAATGAATAAAAACGATCAGGAAACACAGC  
Pdnak(+1)-R CATCAATCCTCTCCTTTCAGTTGGTGGTTCCAAGGTCA  
PdnaK(−1)-F ATTACTCTAGAGCGTGAGACTTGGTGTCAAA XbaI
PdnaK(−2)-F ATTACTCTAGAGGATGGTGCTTTTTGCATC XbaI
PdnaK(−3)-F ATATCTCTAGAGCGGTGGCTCAAAATTGCCTTCA XbaI
PdnaK(−)-R CATCAATCCTCTCCTTTCGTGATTTTAGTACTGTCCAC  
PdnaK()B2-F GAAAGGAGAGGATTGATGAA  
RT-B2-F ACACAGGCTCCGTTGATGAT  
RT-B2-R TTATGGCCCGAAACGTTAAT  
RT-16S-F ACCTGGAGAAGAAGCACCG  
RT-16S-R TCAAGTTATGCCCGTATCG  
  1. The restriction sites are underlined. The RBSs are italicized. The ASs are in boldface
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