Table 2 Primers used in this study
Primers | Sequences (5′–3′) | Restriction sites |
|---|---|---|
R-B2-F | GGGGTTAACAGAAGGAGGGATTGCATGAATAAAAACGATCAGGA | HpaI |
gadB2-R | CGGGATCCTTAACTTCGAACGGTGGTCT | BamHI |
R1-B2-F | CGGGGCTTAAGAAAAGGAGGGATTGCATGAATAAAAACGATCAGGA | AflII |
R2-B2-F | CGGGCTTAAGGGAAGGAGGGATTGCATGAATAAAAACGATCAGGA | AflII |
R3-B2-F | GCCCTTAAGGAAAGGCGAGGATTGCATGAATAAAAACGATCAGGA | AflII |
R4-B2-F | CGGGGCTTAAGGAAAGGAGAGGATTGCATGAATAAAAACGATCAGGA | AflII |
R5-B2-F | CGGGCTTAAGGAAAGGAGGGATTGCATGAATAAAAACGATCAGGA | AflII |
R6-B2-F | CGGGCTTAAGAGGAGGAGGGATTGCATGAATAAAAACGATCAGGA | AflII |
R7-B2-F | CGGGGCTTAAGGAAAGGAGGATTGCATGAATAAAAACGATCAGGA | AflII |
R8-B2-F | CGGGGCTTAAGAGAAAGGAGGGGATTGCATGAATAAAAACGATCAGGA | AflII |
R9-B2-F | CGGGGCTTAAGGAAAGGAGGGGATTGCATGAATAAAAACGATCAGGA | AflII |
R10-B2-F | GGCGCTTAAGGAGAGGAGGGATTGCATGAATAAAAACGATCAGGA | AflII |
R11-B2-F | CGGGGCTTAAGAAGAGGAGGGATTGCATGAATAAAAACGATCAGGA | AflII |
R12-B2-F | CGGGGCTTAAGGGGAGGAGGGATTGCATGAATAAAAACGATCAGGA | AflII |
R4a-B2-F | GCCGGCTTAAGGAAAGGAGAGGATTGATGAATAAAAACGATCAGGA | AflII |
R4b-B2-F | GCCGGCTTAAGGAAAGGAGAGGATTGCAATGAATAAAAACGATCAGGA | AflII |
R4a-B1mut-F | GGAAGATCTGAAAGGAGAGGATTGATGGCTATGTTGTATGGAA | BglII |
gadB1mut-R | AACTGCAGTTAGTGCGTGAACCCGTATTTTTT | PstI |
M-R4a-B1mut-F | ACGCGTCGACGGTTCTGGCAAATATTCTGAAA | SalI |
P sod -F | CTAGTCTAGACTTATGCCCTTCAACCCTACTTA | XbaI |
P sod -R | GCAAGATCTAGATTCGTAGGTTTCCGCACCGAGCATA | XbaI |
P uspA -F | CTAGTCTAGACGAGATGAGATCTTCGAGCT | XbaI |
P uspA -R | GCGGACTCTAGAAAACTTATGACAGGGGTTAAAA | XbaI |
P cspB -F | CTAGTCTAGATAGTCAAGAATTTACCCCCT | XbaI |
P cspB -R | CTAGTCTAGATTGAACACTGCGCACTGAAA | XbaI |
P tuf -F | CATTTCTAGATAAGTGGGGTAGCGGCTTGT | XbaI |
P tuf -R | CCGCTATCTAGAAATTGGTTTTGCTTTCACTG | XbaI |
P gdh -F | CCTAGTCTAGAGAAGAGACTTCATGCAGTTACC | XbaI |
P gdh -R | CCGGTCTAGAACGATTTTAAAGTGTGTATCTG | XbaI |
P dtsR -F | CTAGTCTAGACACGCCCAAAAAGTTTTACC | XbaI |
P dtsR -R | CCTAGTCTAGATGTTTTGAAATCGTAGCGGTA | XbaI |
P odhI -F | CATTTCTAGACGATCACGAGGGGGCACATT | XbaI |
P odhI -R | CCCGGCTCTAGATCTTAACGATTTTCATCATA | XbaI |
P sigB -F | ATATTCTAGATTAACGAAGGCCCCTTCACC | XbaI |
P sigB -R | GTATTCTAGAGGTTCAAGAGGTTCAACGGA | XbaI |
P hmp -F | CTCCTCTAGACGATAAAACGGCATTAAACC | XbaI |
P hmp -R | CGGGTCTAGAGCTAATTTCTACGGATTTAATC | XbaI |
P pqo -F | GTCCTCTAGAAGCAACGACGGAAATCCCAAAA | XbaI |
P pqo -R | GTTCTCTAGATGCCTAACTTGGTGCGACTT | XbaI |
P gapA -F | GCCATCTAGAAAACTATTTAGCGCAAGTGT | XbaI |
P gapA -R | GCCATCTAGATGTAGGAAATGCAATGTGTC | XbaI |
P ilvE -F | ATACTCTAGAGGGGGAGGCATCAAATTGA | XbaI |
P ilvE -R | GCGCTCTAGATCATGGATTTTAAGGTACAC | XbaI |
P cg1417 -F | GTCCTCTAGAGTGTCGTTTTTTGTGCACCTGTTTTG | XbaI |
P cg1417 -R | GGGGTCTAGAGAAAATCCAGGTTAAAAGC | XbaI |
P dnaK -F | CGCGTCTAGAATTGGGTGGTTGAAAATTAG | XbaI |
P dnaK -R | CGCGTCTAGAGTGATTTTAGTACTGTCCA | XbaI |
P clgR -F | ATATTCTAGAAACAAGATGGTCATCCGGTG | XbaI |
P clgR -R | GCTTTCTAGACGTTAGGTTCAACTCCCTTT | XbaI |
P clpB -F | ATATTCTAGATGATTTTTGGCCTCGCGTGG | XbaI |
P clpB -R | CGATTCTAGAGCCAACCTACACAATCAGT | XbaI |
P dnaJ -F | CATTTCTAGATTAGTGGTTTCCGCCGTTGT | XbaI |
P dnaJ -R | CAGGTCTAGAGAGTCTTATATGCGGTGAAT | XbaI |
P sufR -F | CTAGTCTAGATACCTTTGGTTGGCTTAGGG | XbaI |
P sufR -R | CTAGTCTAGATGGTGTCACCTCCTGCTTGA | XbaI |
P trxB1 -F | CTAGTCTAGACCGCAACAATGCCGATTTCA | XbaI |
P trxB1 -R | CGCGTCTAGATTCAAGATTTGTAAGGTCTA | XbaI |
P dnaK -2SD-R | CATTAATCCTCTCCTTTCGAGTTGGTGGTTCCAAGGTCA | |
P dnaK -2SDB2-F | CCACCAACTCGAAAGGAGAGGATTAATGAATAAAAACGATCAGGAAACACAGC | |
Pdnak(+1)-R | CATCAATCCTCTCCTTTCAGTTGGTGGTTCCAAGGTCA | |
PdnaK(−1)-F | ATTACTCTAGAGCGTGAGACTTGGTGTCAAA | XbaI |
PdnaK(−2)-F | ATTACTCTAGAGGATGGTGCTTTTTGCATC | XbaI |
PdnaK(−3)-F | ATATCTCTAGAGCGGTGGCTCAAAATTGCCTTCA | XbaI |
PdnaK(−)-R | CATCAATCCTCTCCTTTCGTGATTTTAGTACTGTCCAC | |
PdnaK()B2-F | GAAAGGAGAGGATTGATGAA | |
RT-B2-F | ACACAGGCTCCGTTGATGAT | |
RT-B2-R | TTATGGCCCGAAACGTTAAT | |
RT-16S-F | ACCTGGAGAAGAAGCACCG | |
RT-16S-R | TCAAGTTATGCCCGTATCG |