Skip to main content

Table 1 Plasmids and strains used in this study

From: Genetic and physiological basis for antibody production by Kluyveromyces marxianus

Plasmids and strains Genotype References
Plasmids
 E02-012 KmP MDH1 _KmINU1ss_scFv_T TDH3 , KmARS7, KmCEN D, kanMX, ori, and AmpR This study
 E02-014 KmP ACO1 _KmINU1ss_scFv_T TDH3 , KmARS7, KmCEN D, kanMX, ori, and AmpR This study
 E02-020 KmP MDH1 _Scα-MFss_scFv_T TDH3 , KmARS7, KmCEN D, kanMX, ori, and AmpR This study
 E02-022 KmP ACO1 _Scα-MFss_scFv_T TDH3 , KmARS7, KmCEN D, kanMX, ori, and AmpR This study
 Cas9_Base ScP PDC1 _Cas9_T TDH3 , KmARS7, KmCEN D, kanMX, ori, and AmpR Nambu-Nishida et al. (2017)
 E02-025 KmP SNR52 _target_gRNA-1_sgRNA_T sup4 cassette and KmP SNR52 _target_gRNA-3_sgRNA_T sup4 cassette in Cas9_Base This study
 E02-026 KmP SNR52 _target_gRNA-1_sgRNA_T sup4 cassette and KmP SNR52 _target_gRNA-2_sgRNA_T sup4 cassette in Cas9_Base This study
Strains
 1 (NBRC1777) Wild-type (WT) NITE
Biological Resource Center, Japan
 Nej1° Nej1 disrupted by C to T point mutation at posision 13 Nambu-Nishida et al. (2017)
 Dnl4° Dnl4 disrupted by G to A point mutation at posision 44 Nambu-Nishida et al. (2017)
 2 (Km02-026) WT/E02-012 This study
 3 (Km02-032) WT/E02-014 This study
 4 (Km02-050) WT/E02-020 This study
 5 (Km02-056) WT/E02-022 This study
 Δinu1 (Km02-063) Nej1°inu1 This study
 6 (Km02-064) Nej1°/inu1::P INU1 _scFv This study
 7 (Km02-065) Dnl4°/inu1::P INU1 _scFv This study
 8 (Km02-066) Nej1°/inu1::/E02-020 This study
 BY4741 MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ATCC (Brachmann et al. 1998)