Table 1 Plasmids and strains used in this study
From: Genetic and physiological basis for antibody production by Kluyveromyces marxianus
Plasmids and strains | Genotype | References |
|---|---|---|
Plasmids | ||
E02-012 | KmP MDH1 _KmINU1ss_scFv_T TDH3 , KmARS7, KmCEN D, kanMX, ori, and AmpR | This study |
E02-014 | KmP ACO1 _KmINU1ss_scFv_T TDH3 , KmARS7, KmCEN D, kanMX, ori, and AmpR | This study |
E02-020 | KmP MDH1 _Scα-MFss_scFv_T TDH3 , KmARS7, KmCEN D, kanMX, ori, and AmpR | This study |
E02-022 | KmP ACO1 _Scα-MFss_scFv_T TDH3 , KmARS7, KmCEN D, kanMX, ori, and AmpR | This study |
Cas9_Base | ScP PDC1 _Cas9_T TDH3 , KmARS7, KmCEN D, kanMX, ori, and AmpR | Nambu-Nishida et al. (2017) |
E02-025 | KmP SNR52 _target_gRNA-1_sgRNA_T sup4 cassette and KmP SNR52 _target_gRNA-3_sgRNA_T sup4 cassette in Cas9_Base | This study |
E02-026 | KmP SNR52 _target_gRNA-1_sgRNA_T sup4 cassette and KmP SNR52 _target_gRNA-2_sgRNA_T sup4 cassette in Cas9_Base | This study |
Strains | ||
1 (NBRC1777) | Wild-type (WT) | NITE Biological Resource Center, Japan |
Nej1° | Nej1 disrupted by C to T point mutation at posision 13 | Nambu-Nishida et al. (2017) |
Dnl4° | Dnl4 disrupted by G to A point mutation at posision 44 | Nambu-Nishida et al. (2017) |
2 (Km02-026) | WT/E02-012 | This study |
3 (Km02-032) | WT/E02-014 | This study |
4 (Km02-050) | WT/E02-020 | This study |
5 (Km02-056) | WT/E02-022 | This study |
Δinu1 (Km02-063) | Nej1°/Δinu1 | This study |
6 (Km02-064) | Nej1°/inu1::P INU1 _scFv | This study |
7 (Km02-065) | Dnl4°/inu1::P INU1 _scFv | This study |
8 (Km02-066) | Nej1°/inu1::/E02-020 | This study |
BY4741 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | ATCC (Brachmann et al. 1998) |