Fig. 4From: Genetic and physiological basis for antibody production by Kluyveromyces marxianusImmunoreactivity of secreted scFv analyzed by ELISA. Strains were cultured in YPD or YPX in the absence or presence of MgSO4 at 20 °C (a) or 30 °C (b). Spent culture medium was harvested at 72 h and subjected to ELISA using lysozyme as an antigen for scFv. Values are presented as mean ± SEM from three independent experimentsBack to article page