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Fig. 3 | AMB Express

Fig. 3

From: Genetic and physiological basis for antibody production by Kluyveromyces marxianus

Fig. 3

INU1 deletion analyzed by SDS-PAGE and secretion of scFv analyzed by immunoblotting. a Schematic of the general construction of scFv-expression plasmid. kanMX: G418 resistance gene, KmCEN-D: centromere sequence of K. marxianus, KmARS7: autonomous replication sequence of K. marxianus. b Combinations of promoters and signal peptides tested. c Stains were cultivated for 48 h in YPD with or without G418, and SDS-PAGE was used to analyze the spent culture medium from strains No. 1 (wild-type) (lane 1), No. 6 (Km02-064) (lane 2), No. 7 (Km02-065) (lane 3), and No. 8 (Km02-066) (lane 4). Sizes of the molecular weight marker (lane M) are indicated on the left. d Immunoblot of spent culture medium from strains cultivated for 72 h in YPD with or without G418. Lane numbers correspond to those in panel (b)

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