Fig. 2
From: Genetic and physiological basis for antibody production by Kluyveromyces marxianus
INU1 deletion and replacement analyzed by agarose gel electrophoresis. Sequences from the transformed cells were PCR-amplified and analyzed by agarose gel electrophoresis. A pair of primers (P_Km01-010 + P_Km01-011) flanking the INU1 locus were used to amplify intervening sequences. The resulting amplicons from the wild-type strain (lane 1), inu1-deleted strain Δinu1 (Km02-063) (lane 2), or scFv integration strains No. 6 (Km02-064) (lane 3) and No. 7 (Km02-065) (lane 4) matched the expected fragment sizes of 3057, 492, 2184, and 2184 bp, respectively. DNA ladder markers (1 Kb and 100 bp) are provided as size standards (lanes M1 and M2, respectively)