Fig. 1

Design of INU1 disruption and replacement. a A schematic of the INU1 locus and its deletion is shown. Reverse complement sequences of target (red) and PAM (shadowed) are shown on the reference sequence. The lower part of the panel shows an alignment of the sequence from the deletion strain with the sequence of the wild-type locus. b A schematic of the INU1 replacement by a fragment encoding scFv is shown. The mutated PAM sequence and its reverse complement sequence are highlighted in yellow. Mutated bases are indicated as small red letters. The scFv-encoding fragment also encodes the INU1 secretion signal peptide (blue box) and a 6-histidine tag (red box) in-frame with the expressed ORF