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AMB Express
Fig. 3 | AMB Express

Fig. 3

From: Efficient production of secretory Streptomyces clavuligerus β-lactamase inhibitory protein (BLIP) in Pichia pastoris

Fig. 3

Expression of secretory BLIP in P. pastoris. a As revealed by a coomassie-blue stained SDS-PAGE gel, highly pure BLIP was found to be present in the culture media collected 24, 48 and 72 h after induction with 2% MeOH. b As analyzed by ESI–MS, the measured mass of the secretory BLIP (peak A) was 18,219 which matched the calculated mass of EAEAEF-mature BLIP (18,219.25). Peak B with molecular mass of 18,241 corresponded to the sodium adduct of the secretory BLIP

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