Fig. 4

Subcellular localization of the HOG1::GFP fusion protein in response to changes in osmotic pressure (a), and after illumination (b) of the cells with blue light. Activation of Sln1 signaling was initiated with: a a change in osmotic pressure elicited by addition of 0.4 M NaCl (final concentration) to the cell suspension, and b illumination by exposure of the cells to blue light (200 μE incident intensity, 450 nm LED light). The strain used for a was: ∆HOG1, pRS416-HOG1::GFP; and for b: ∆HOG1, pRS416-HOG1::GFP, ∆Sln1, pRS325ActC1LEU