Fig. 3

Effect of illumination on the Skn7-dependent expression of the OCH1-lacZ transcriptional fusion (i.e. the mannosyl-transferase gene fused with the reading frame encoding β-galactosidase) in a S. cerevisiae strain with the wild-type sln1 gene, and in a strain with sln1 replaced by the gene encoding the C1-histidine kinase fusion protein. Cells were incubated either in the presence of light (white bars) or in the dark (grey bars). The level of expression of the OCH1-lacZ fusion is deduced from the specific β-galactosidase activity, expressed in Miller units. Error bars indicate standard deviations calculated from three independent biological experiments