Effect of cultivation mode on the production of docosahexaenoic acid by Tisochrysis lutea

AMB Express

Table 4 DHA and TFA production from T. lutea in the literature and in this study

Culture mode	Aeration	DHA/TFA (%)	DHA content (mg/g)	DHA production (mg/L)	TFA content (% DW) and production (mg/L)	Refs.
Autotrophy	Air	14%	21 mg/g	NR	15%	Tzovenis et al. (1997)
Autotrophy	NR	12.2%	18.1 mg/g	5.8 mg/L	14.74% and 47.5 mg/L	Tzovenis et al. (2003)
Autotrophy	No	8–13%	15–17 mg/g	NR	13%	Mulders et al. (2013)
Autotrophy	Air with 1% CO₂	8.5%	NR	16.1 mg/L	224 mg/L	Nalder et al. (2015)
Autotrophy	Air with 1% CO₂	8.2%	NR	41 mg/L	500 mg/L	Rasdi and Qin (2015)
Autotrophy Mixotrophy	Air	Autotrophy 10.1% Mixotrophy 8.6%	Autotrophy 22.22 mg/g Mixotrophy 16.59 mg/g	Autotrophy 8.36 mg/L Mixotrophy 13.94 mg/L	Autotrophy 22% and 83.6 mg/L Mixotrophy 19.3% and 162.1 mg/L	Alkhamis and Qin (2016)
Atotrophy Mixotrophy Heterotrophy	Air with 4% CO₂	Autotrophy 12.7% Mixotrophy 13.8% Heterotrophy 9.4%	Autotrophy 33.18 mg/g Mixotrophy 37.98 mg/g Heterotrophy 7.64 mg/g	Autotrophy 39.50 mg/L Mixotrophy 55.69 mg/L Heterotrophy 2.43 mg/L	Autotrophy 26.1% and 310.7 mg/L Mixotrophy 28.0% and 408.5 mg/L Heterotrophy 8.1% and 24.7 mg/L	This study