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AMB Express

Table 1 Purity and concentration of the extracted genomic DNA

From: A modified method for genomic DNA extraction from the fish intestinal microflora

Method

IT (lysozyme/RNase)

DNA (ng/µL)

DNA (ng/µL) range

A260/280

A260/280 range

DNA integrity

CLU

37 °C/37 °C

309.80 ± 66.79

133–448

2.083 ± 0.141

1.819–2.423

++

60 °C/30 °C

282.80 ± 85.09

50–478

2.020 ± 0.161

1.701–2.407

++

ZBC

NA

1002.60 ± 365.15

300–2352

2.074 ± 0.147

1.712–2.413

+

QIA

NA

161.60 ± 54.35

82.5–373

2.089 ± 0.096

1.810–2.224

++

  1. Values are represented as the mean ± SE; DNA integrity was assayed by agarose electrophoresis and indicated as good (++) or sufficient (+) based on the band clarity, density and presence of smearing
  2. NA not available, IT incubation temperature
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