Table 2 GC–MS analysis of the methylated products of sugar beet arabinan and its enzymatic product
From: Cloning and expression of a novel α-1,3-arabinofuranosidase from Penicillium oxalicum sp. 68
Methylated sugars | Type of linkage | Molar ratio (before) | Molar ratio (after) | Mass fragments (m/z) |
|---|---|---|---|---|
1,4,5-Tri-O-actyl-2,3-di-O-methyl-arabinitol | 1,5-Linked Araf | 39.7 | 58.9 | 87,101,117,129,189 |
1,3,4-Tri-O-actyl-2,5-di-O-methyl-arabinitol | 1,3,5-Linked Araf | 24.0 | 1.3 | 85,99,117,127,159,201,261 |
1,2,4-Tri-O-actyl-3,5-di-O-methyl-arabinitol | 1,2,5-Linked Araf | 1.0 | 0.8 | 87,101,117,129,189,203 |
1,2,3,4,5-Penta-O-actyl-arabinitol | 1,2,3,5-Linked Araf | 4.2 | 3.4 | 85,115,127,145,187,233,289 |
1,4-Di-O-acetyl-2,3,5-tri-O-methyl-arabinitol | Terminal Araf | 28.5 | 7.8 | 87,101,117,129,161 |
1,5-Di-O-acetyl-1-deuterio-2,3,4,6-tetra-O-methyl-d-galactitol | Terminal Galp | 1.3 | 1.2 | 71,87,101,117,129 |
1,5,6-Tri-O-acetyl-1-deuterio-2,3,4-tri-O-methyl-d-galactitol | 1,6-Linked Galp | 1.5 | 1.0 | 87,101,117,129,143,161,189,233 |