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AMB Express

Table 1 Summary of purification of recombinant PoAbf62A

From: Cloning and expression of a novel α-1,3-arabinofuranosidase from Penicillium oxalicum sp. 68

Purification step

Volume (ml)

Total protein (mg)a

Activity (U)b

Specific activity (U/mg)

Purification (fold)

Yield (%)

(NH4)2SO4

20

16.7

3.8

0.56

1.0

100

Phenyl Sepharose 6 Fast Flow (high sub)

2

2.2

1.75

0.80

1.43

46

  1. aProtein was quantified according to the Bradford method using bovine serum albumin (BSA) as standard
  2. bThe activity was reported as activity on pNPαAraf
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