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AMB Express
Fig. 1 | AMB Express

Fig. 1

From: Effect of CBM1 and linker region on enzymatic properties of a novel thermostable dimeric GH10 xylanase (Xyn10A) from filamentous fungus Aspergillus fumigatus Z5

Fig. 1

SDS-PAGE and zymogram analysis of the expressed xylanases. a These crude xylanases, Xyn10A, Xyn10AdC, Xyn10AdLC, Xyn10B and Xyn10BaLC (expressed in P. pastoris X33) were buffered and separated by SDS-PAGE but without pre-heating. b These crude xylanases, Xyn10A, Xyn10AdC, Xyn10AdLC, Xyn10B and Xyn10BaLC (expressed in P. pastoris X33) were buffered and separated by SDS-PAGE with heating treatment at 100 °C for 5 min before loading. c Zymogram analysis for Xyn10A, Xyn10AdC and Xyn10AdLC. d SDS-PAGE of the purified xylanases (Xyn10A, Xyn10AdC, Xyn10AdLC, Xyn10B and Xyn10BaLC). M, protein marker

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