Fig. 2
From: Expression of recombinant HBD3 protein that reduces Mycobacterial infection capacity
Expression and identification of rHBD3 protein. a Effects of different post induction times on the expression of target fusion protein with BL21 (DE3) pGEX-5X-HBD3. Soluble and insoluble proteins were analyzed at various post induction times by using SDS-PAGE (S soluble fractions, I insoluble fractions). b SDS-PAGE analysis of collected samples of purified fusion protein. M is the protein marker. Lanes are all the purified fusion protein. c SDS-PAGE analysis of protein fragment that has been digested by Factor Xa. M is the protein marker. Lane 1 is the purified recombination HBD3, lane 2 is the digested protein fragments lane 3 is pure GST-HBD3 protein, lane 4 is all protein. d Western blot analysis of recombination HBD3. Lanes are all the purified rHBD3