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Table 1 Biochemical properties of TsPOx, its mutants, and other pyranose oxidases

From: Biochemical and mutational analyses of a Trametes pyranose oxidase and comparison of its mutants in breadmaking

Enzyme

Organism

Optimal pH

Optimal temperature (°C)

Specific activity (U/mg)

kcat (s−1)

Km (mM)

k cat /Km (M−1 s−1)

References

TsPOx

Trametes sp.

6.5

50

6.4 ± 0.8

6.1 ± 0.2

1.2 ± 0.1

5083

This study

 E539K

 

6.0

60

5.3 ± 0.2

7.6 ± 0.5

0.7 ± 0.0

10,857

 

 K312E

 

5.5

50

10.2 ± 1.1

8.5 ± 0.0

0.8 ± 0.1

10,625

 

 K312E/E539K

 

6.0

60

6.2 ± 0.1

7.6 ± 0.2

0.6 ± 0.0

12,667

 

 T166A/E539K

 

6.0

50

2.4 ± 0.0

2.9 ± 0.0

0.5 ± 0.0

5800

 

PROD

Trametes versicolor

7.0‒7.5

50

12.8

59.9

1.4

42,785

Nishimura et al. (1996)

 E542K

 

N/A

55

13.3

70.6

0.7

100,857

Masuda-Nishimura et al. (1999)

AoPOx

Aspergillus oryzae

N/A

N/A

0.58

1.48

2.86

517

Pisanelli et al. (2012a)

AnPOx

Aspergillus nidulans

N/A

N/A

1.16

35.44

1.77

20,022

Pisanelli et al. (2012b)

Pyranose oxidase

Tricholoma matsutake

7.7‒8.0

50

26.6

111

1.28

86,718

Takakura and Kuwata (2003)

P2Ox

Peniophora gigantea

4.5‒6.0

44

0.29

11.9

0.8

14,875

Bastian et al. (2005)

 E540K

 

4.5‒6.0

N/A

13.4

28.5

0.6

47,500

 

 E540K/312E

 

N/A

50

N/A

107.6

0.4

269,000

 

P2OxA

Peniophora sp.

5.0‒6.5

50

2.1

9.4

5.0

1880

Heckmann-Pohl et al. (2006)

 E542K

 

5.0‒6.5

58

4.34

19.4

1.0

19,400

 

 T158A/E542K

 

5.5‒6.0

51

29.54

133.48

0.47

284,000