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Table 1 Biochemical properties of TsPOx, its mutants, and other pyranose oxidases

From: Biochemical and mutational analyses of a Trametes pyranose oxidase and comparison of its mutants in breadmaking

Enzyme Organism Optimal pH Optimal temperature (°C) Specific activity (U/mg) kcat (s−1) Km (mM) k cat /Km (M−1 s−1) References
TsPOx Trametes sp. 6.5 50 6.4 ± 0.8 6.1 ± 0.2 1.2 ± 0.1 5083 This study
 E539K   6.0 60 5.3 ± 0.2 7.6 ± 0.5 0.7 ± 0.0 10,857  
 K312E   5.5 50 10.2 ± 1.1 8.5 ± 0.0 0.8 ± 0.1 10,625  
 K312E/E539K   6.0 60 6.2 ± 0.1 7.6 ± 0.2 0.6 ± 0.0 12,667  
 T166A/E539K   6.0 50 2.4 ± 0.0 2.9 ± 0.0 0.5 ± 0.0 5800  
PROD Trametes versicolor 7.0‒7.5 50 12.8 59.9 1.4 42,785 Nishimura et al. (1996)
 E542K   N/A 55 13.3 70.6 0.7 100,857 Masuda-Nishimura et al. (1999)
AoPOx Aspergillus oryzae N/A N/A 0.58 1.48 2.86 517 Pisanelli et al. (2012a)
AnPOx Aspergillus nidulans N/A N/A 1.16 35.44 1.77 20,022 Pisanelli et al. (2012b)
Pyranose oxidase Tricholoma matsutake 7.7‒8.0 50 26.6 111 1.28 86,718 Takakura and Kuwata (2003)
P2Ox Peniophora gigantea 4.5‒6.0 44 0.29 11.9 0.8 14,875 Bastian et al. (2005)
 E540K   4.5‒6.0 N/A 13.4 28.5 0.6 47,500  
 E540K/312E   N/A 50 N/A 107.6 0.4 269,000  
P2OxA Peniophora sp. 5.0‒6.5 50 2.1 9.4 5.0 1880 Heckmann-Pohl et al. (2006)
 E542K   5.0‒6.5 58 4.34 19.4 1.0 19,400  
 T158A/E542K   5.5‒6.0 51 29.54 133.48 0.47 284,000