Post-ruminal effects of rumen-protected methionine supplementation with low protein diet using long-term simulation and in vitro digestibility technique

AMB Express

Table 5 Low dietary protein with rumen-protected methionine supplementation effects on daily production of total and individual volatile fatty acids using an in vitro abomasum digestibility technique (means; n = 4)

Items	Treatments				SEM	P value
Items	HP	LP	LPLM	LPHM	SEM	P value
Total VFA (mM)	52.81^a	45.07^b	48.74^a,b	51.92^a	1.23	0.03
Acetate (mM)	29.79^a	25.34^b	27.97^a,b	29.07^a,b	0.70	0.04
Propionate (mM)	11.8^a	9.74^b	10.40^a,b	11.72^a	0.36	0.04
Isobutyrate (mM)	0.43	0.41	0.38	0.41	0.01	0.76
Butyrate (mM)	6.76^a	6.17^b	6.30^b	6.64^a	0.09	0.00
Isovalerate (mM)	1.33	1.47	1.40	1.44	0.04	0.81
Valerate (mM)	2.70	1.94	2.29	2.63	0.13	0.11
Acetate:propionate ratio	2.52	2.60	2.69	2.48	0.03	0.05

VFA volatile fatty acids, SEM standard error of the mean
^a,b Superscripts values within the same row are significantly different at (P < 0.05)