Post-ruminal effects of rumen-protected methionine supplementation with low protein diet using long-term simulation and in vitro digestibility technique

AMB Express

Table 1 Ingredients and chemical composition of the experimental diets fed by donor animals and supplied daily to each fermenter (DM basis, g/day)

Items	Treatments
Items	HP	LP	LPLM	LPHM
Ingredients supply (g/day)
Corn silage	3.65	3.87	3.87	3.87
Alfalfa hay	2.03	0.78	0.78	0.78
Wheat straw	3.06	4.28	4.27	4.27
Ground corn	3.25	4.33	4.33	4.33
Wheat bran	1.56	0.94	0.94	0.93
Soybean meal	1.41	2.01	2.01	2.01
Cottonseed meal	1.92	0.76	0.76	0.76
Corn germ meal	2.31	2.11	2.11	2.11
Limestone	0.21	0.18	0.18	0.18
Dicalcium phosphate	0.00	0.10	0.10	0.10
NaCl	0.21	0.24	0.24	0.24
NaHCO₃	0.04	0.04	0.04	0.04
Premix^a	0.36	0.36	0.36	0.36
RPM	0.0000	0.0000	0.0021	0.0200
Chemical composition
Dry matter (g/day)	9.03	8.90	8.92	8.94
Crude protein (g/day)	1.63	1.42	1.42	1.43
aNeutral detergent fiber (g/day)	4.71	4.72	4.71	4.70
Acid detergent fiber (g/day)	2.12	2.11	2.05	2.12
Ether extract (g/day)	0.29	0.28	0.28	0.28
Gross energy (MJ/day)	0.16	0.16	0.16	0.17

^aPremix (contains): 9.80 mg Zn, 10.5 mg Cu, 12.00 mg Mn, 0.32 mg I, 0.15 mg Se, 0.11 mg Co, 500 IU vitamin D3, 40 IU vitamin E, and 2500 IU vitamin A, per kilogram of TMR on DM basis