Table 2 Strains used in the present study
From: Exploring d-xylose oxidation in Saccharomyces cerevisiae through the Weimberg pathway
Strain name | Relevant genotype | Encoded enzymes introduced into yeast | References |
|---|---|---|---|
CEN.PK 113-7D | MATa URA3 HIS3 LEU2 TRP1 MAL2-8C SUC2 | – | Entian and Kötter (2007) |
TMB4545 | VAC17/MRC1::TDH3p-GAL2N376F-CYC1t-FBA1p-TAL-PDC1t-TPI1p-TKL-CPS1t, X-2::XYL-XDH-XKS1; XI-5::XYL-XDH-XKS1, XII-4::XYL-XDH-XKS1 | XR, XDH (with an optimized PPP) | Unpublished |
TMB4511 | CEN.PK 113-7D, Δgre3::AGS7 | XylB, XylC | This study |
TMB4512 | CEN.PK 113-7D, Δgre3::xylB | XylB | This study |
TMB4515 | CEN.PK 113-7D, vac17mrc1::AGS8 | XylD, XylX, XylA | This study |
TMB4520 | CEN.PK 113-7D, Δgre3::AGS7, vac17mrc1::AGS8 | XylB, XylC, XylD, XylX, XylA | This study |
TMB4526 | CEN.PK 113-7D, Δgre3::AGS7, vac17mrc1::AGS8H | XylB, XylC, XAD_Hv, XylX, XylA | This study |
TMB4530 | CEN.PK 113-7D, Δgre3::AGS8B | XylB, XylD, XylX, XylA | This study |
TMB4531 | CEN.PK 113-7D, Δgre3::AGS8HB | XylB, XAD_Hv, XylX, XylA | This study |
TMB4569 | CEN.PK 113-7D, Δgre3::AGS8HB, ΔxylD::xylD_Bc | XylB, XylD_Bc, XylX, XylA | This study |
TMB4570 | CEN.PK 113-7D, Δgre3::AGS8HB, ΔxylD::yjhG_Ec | XylB, YjhG_Ec, XylX, XylA | This study |
TMB4571 | CEN.PK 113-7D, Δgre3::AGS8HB, ΔxylD::xylD_El | XylB, XylD_El, XylX, XylA | This study |