Fig. 6

Reverse-transcriptase PCR analysis to verify gene expression of Weimberg pathway genes. RNA from five different strains exponentially growing on d-glucose was purified and used for cDNA synthesis. Reverse-transcriptase PCR was performed on the cDNA using primers specific for each Weimberg pathway gene. Amplification of xylB, xylA and xylX was carried out in all strains while different xylDs was amplified in each individual strain as follows: xylD from Caulobacter crescentus in TMB4530, xad from Haloferax volcanii in TMB4531, xylD from Burkholderia cenocepacia in TMB4569, yjhG from Escherichia coli in TMB4570 and xylD from Ellin329 isolate in TMB4571. CEN.PK 113-7D (WT) was used as negative control. Before cDNA synthesis the RNA purity (i.e. removal of DNA) was examined using a control PCR that amplifies the constitutively expressed gene PFY1 encoding Profilin