Strains and plasmids | Relevant characteristics | Sources or references |
---|---|---|
Strains | ||
E. coli DH5α | Clone host strain | Transgen |
C. glutamicum S9114 | Industrial strain for glutamate production | Mei et al. (2016) |
CO | C. glutamicum S9114△ncgl1221△lysE△putP△argR | Our lab |
CO-1 | CO derivative with in-frame deletion of argF | This study |
CO-2 | CO derivative with replacement of RBS10 and A1G in argF | This study |
CO-3 | CO derivative with replacement of RBS50 and start A1G in argF | This study |
CO-4 | CO derivative with replacement of RBS100 A1G in argF | This study |
CO-5 | CO derivative with replacement of RBS500 and A1G in argF | This study |
CO-6 | CO derivative with insertion of terminator T1 in front of argF | This study |
CO-7 | CO derivative with insertion of terminator T2 in front of argF | This study |
CO-8 | CO derivative with insertion of terminator T3 in front of argF | This study |
CO-9 | CO derivative with insertion of terminator T4 in front of argF | This study |
CO-10 | CO derivative with insertion of terminator T5 in front of argF | This study |
CO-11 | CO derivative with insertion of terminator T6 in front of argF | This study |
CO-12 | CO derivative with insertion of terminator T7 in front of argF | This study |
CO-13 | CO derivative with insertion of terminator T8 in front of argF | This study |
CO-14 | CO derivative with insertion of terminator T9 in front of argF | This study |
CO-15 | CO derivative with insertion of terminator T10 in front of argF | This study |
CO-16 | CO derivative with insertion of terminator T11 in front of argF | This study |
CO-17 | CO derivative with insertion of terminator T12 in front of argF | This study |
CO-18 | CO derivative with insertion of terminator T13 in front of argF | This study |
CO-19 | CO derivative with insertion of terminator T14 in front of argF | This study |
CO-20 | CO derivative with insertion of terminator T15 in front of argF | This study |
CO-21 | CO derivative with insertion of terminator T16 in front of argF | This study |
CO-22 | CO derivative with insertion of terminator T17 in front of argF | This study |
CO-23 | CO derivative with insertion of terminator T18 in front of argF | This study |
Plasmids | ||
pK18mobsacB | Mobilizable vector, allows for selection of double crossover in C. glutamicum, KmR, sacB | Zhang et al. (2015) |
pXMJ19 | A shuttle expression vector, CmR | Jakoby et al. (1999) |
pK18-△argF | A derivative of pK18mobsacB, harboring △argF fragment | This study |
pK18-argF10-G | A derivative of pK18mobsacB, harboring the fragment of argF (10 au) RBS change and start codon replacement with GTG | This study |
pK18-argF50-G | A derivative of pK18mobsacB, harboring the fragment of argF (50 au) RBS change and start codon replacement with GTG | This study |
pK18-argF100-G | A derivative of pK18mobsacB, harboring the fragment of argF (100 au) RBS change and start codon replacement with GTG | This study |
pK18-argF500-G | A derivative of pK18mobsacB, harboring the fragment of argF (500 au) RBS change and start codon replacement with GTG | This study |
pK18-T1 | A derivative of pK18mobsacB, harboring the fragment of inserting T1 terminator in front of argF | This study |
pK18-T2 | A derivative of pK18mobsacB, harboring the fragment of inserting T2 terminator in front of argF | This study |
pK18-T3 | A derivative of pK18mobsacB, harboring the fragment of inserting T3 terminator in front of argF | This study |
pK18-T4 | A derivative of pK18mobsacB, harboring the fragment of inserting T4 terminator in front of argF | This study |
pK18-T5 | A derivative of pK18mobsacB, harboring the fragment of inserting T5 terminator in front of argF | This study |
pK18-T6 | A derivative of pK18mobsacB, harboring the fragment of inserting T6 terminator in front of argF | This study |
pK18-T7 | A derivative of pK18mobsacB, harboring the fragment of inserting T7 terminator in front of argF | This study |
pK18-T8 | A derivative of pK18mobsacB, harboring the fragment of inserting T8 terminator in front of argF | This study |
pK18-T9 | A derivative of pK18mobsacB, harboring the fragment of inserting T9 terminator in front of argF | This study |
pK18-T10 | A derivative of pK18mobsacB, harboring the fragment of inserting T10 terminator in front of argF | This study |
pK18-T11 | A derivative of pK18mobsacB, harboring the fragment of inserting T11 terminator in front of argF | This study |
pK18-T12 | A derivative of pK18mobsacB, harboring the fragment of inserting T12 terminator in front of argF | This study |
pK18-T13 | A derivative of pK18mobsacB, harboring the fragment of inserting T13 terminator in front of argF | This study |
pK18-T14 | A derivative of pK18mobsacB, harboring the fragment of inserting T14 terminator in front of argF | This study |
pK18-T15 | A derivative of pK18mobsacB, harboring the fragment of inserting T15 terminator in front of argF | This study |
pK18-T16 | A derivative of pK18mobsacB, harboring the fragment of inserting T16 terminator in front of argF | This study |
pK18-T17 | A derivative of pK18mobsacB, harboring the fragment of inserting T17 terminator in front of argF | This study |
pK18-T18 | A derivative of pK18mobsacB, harboring the fragment of inserting T18 terminator in front of argF | This study |