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Table 1 Strains and plasmids used in this study

From: Improvement of l-ornithine production by attenuation of argF in engineered Corynebacterium glutamicum S9114

Strains and plasmids Relevant characteristics Sources or references
Strains
 E. coli DH5α Clone host strain Transgen
 C. glutamicum S9114 Industrial strain for glutamate production Mei et al. (2016)
 CO C. glutamicum S9114ncgl1221lysEputPargR Our lab
 CO-1 CO derivative with in-frame deletion of argF This study
 CO-2 CO derivative with replacement of RBS10 and A1G in argF This study
 CO-3 CO derivative with replacement of RBS50 and start A1G in argF This study
 CO-4 CO derivative with replacement of RBS100 A1G in argF This study
 CO-5 CO derivative with replacement of RBS500 and A1G in argF This study
 CO-6 CO derivative with insertion of terminator T1 in front of argF This study
 CO-7 CO derivative with insertion of terminator T2 in front of argF This study
 CO-8 CO derivative with insertion of terminator T3 in front of argF This study
 CO-9 CO derivative with insertion of terminator T4 in front of argF This study
 CO-10 CO derivative with insertion of terminator T5 in front of argF This study
 CO-11 CO derivative with insertion of terminator T6 in front of argF This study
 CO-12 CO derivative with insertion of terminator T7 in front of argF This study
 CO-13 CO derivative with insertion of terminator T8 in front of argF This study
 CO-14 CO derivative with insertion of terminator T9 in front of argF This study
 CO-15 CO derivative with insertion of terminator T10 in front of argF This study
 CO-16 CO derivative with insertion of terminator T11 in front of argF This study
 CO-17 CO derivative with insertion of terminator T12 in front of argF This study
 CO-18 CO derivative with insertion of terminator T13 in front of argF This study
 CO-19 CO derivative with insertion of terminator T14 in front of argF This study
 CO-20 CO derivative with insertion of terminator T15 in front of argF This study
 CO-21 CO derivative with insertion of terminator T16 in front of argF This study
 CO-22 CO derivative with insertion of terminator T17 in front of argF This study
 CO-23 CO derivative with insertion of terminator T18 in front of argF This study
Plasmids
 pK18mobsacB Mobilizable vector, allows for selection of double crossover in C. glutamicum, KmR, sacB Zhang et al. (2015)
 pXMJ19 A shuttle expression vector, CmR Jakoby et al. (1999)
pK18-argF A derivative of pK18mobsacB, harboring argF fragment This study
 pK18-argF10-G A derivative of pK18mobsacB, harboring the fragment of argF (10 au) RBS change and start codon replacement with GTG This study
 pK18-argF50-G A derivative of pK18mobsacB, harboring the fragment of argF (50 au) RBS change and start codon replacement with GTG This study
 pK18-argF100-G A derivative of pK18mobsacB, harboring the fragment of argF (100 au) RBS change and start codon replacement with GTG This study
 pK18-argF500-G A derivative of pK18mobsacB, harboring the fragment of argF (500 au) RBS change and start codon replacement with GTG This study
 pK18-T1 A derivative of pK18mobsacB, harboring the fragment of inserting T1 terminator in front of argF This study
 pK18-T2 A derivative of pK18mobsacB, harboring the fragment of inserting T2 terminator in front of argF This study
 pK18-T3 A derivative of pK18mobsacB, harboring the fragment of inserting T3 terminator in front of argF This study
 pK18-T4 A derivative of pK18mobsacB, harboring the fragment of inserting T4 terminator in front of argF This study
 pK18-T5 A derivative of pK18mobsacB, harboring the fragment of inserting T5 terminator in front of argF This study
 pK18-T6 A derivative of pK18mobsacB, harboring the fragment of inserting T6 terminator in front of argF This study
 pK18-T7 A derivative of pK18mobsacB, harboring the fragment of inserting T7 terminator in front of argF This study
 pK18-T8 A derivative of pK18mobsacB, harboring the fragment of inserting T8 terminator in front of argF This study
 pK18-T9 A derivative of pK18mobsacB, harboring the fragment of inserting T9 terminator in front of argF This study
 pK18-T10 A derivative of pK18mobsacB, harboring the fragment of inserting T10 terminator in front of argF This study
 pK18-T11 A derivative of pK18mobsacB, harboring the fragment of inserting T11 terminator in front of argF This study
 pK18-T12 A derivative of pK18mobsacB, harboring the fragment of inserting T12 terminator in front of argF This study
 pK18-T13 A derivative of pK18mobsacB, harboring the fragment of inserting T13 terminator in front of argF This study
 pK18-T14 A derivative of pK18mobsacB, harboring the fragment of inserting T14 terminator in front of argF This study
 pK18-T15 A derivative of pK18mobsacB, harboring the fragment of inserting T15 terminator in front of argF This study
 pK18-T16 A derivative of pK18mobsacB, harboring the fragment of inserting T16 terminator in front of argF This study
 pK18-T17 A derivative of pK18mobsacB, harboring the fragment of inserting T17 terminator in front of argF This study
 pK18-T18 A derivative of pK18mobsacB, harboring the fragment of inserting T18 terminator in front of argF This study
  1. Superscript ‘‘R’’ indicates resistance to the following antibiotics: Km kanamycin, Cm chloramphenicol