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Table 6 Verification for spore production after two-step RSM optimization procedures

From: Rapid optimization of spore production from Bacillus amyloliquefaciens in submerged cultures based on dipicolinic acid fluorimetry assay

Optimization procedures DPA fluorimetry assay Plate counting assay
Predicted fluorescence intensity (AU) Observed fluorescence intensity (AU) Calculated colony concentrations (CFU/mL) Measured colony concentrations (CFU/mL)
Control 98.6 ± 8.9 (9.01 ± 0.03) × 108 (9.10 ± 0.28) × 108
Metal ion-optimized RSM 300.2 303.3 ± 22.4 (2.94 ± 0.13) × 109 (3.10 ± 1.41) × 109
Raw material-optimized RSM 802.0 803.3 ± 28.3 (7.94 ± 0.20) × 109 (8.05 ± 0.70) × 109