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AMB Express

Table 6 Verification for spore production after two-step RSM optimization procedures

From: Rapid optimization of spore production from Bacillus amyloliquefaciens in submerged cultures based on dipicolinic acid fluorimetry assay

Optimization procedures

DPA fluorimetry assay

Plate counting assay

Predicted fluorescence intensity (AU)

Observed fluorescence intensity (AU)

Calculated colony concentrations (CFU/mL)

Measured colony concentrations (CFU/mL)

Control

–

98.6 ± 8.9

(9.01 ± 0.03) × 108

(9.10 ± 0.28) × 108

Metal ion-optimized RSM

300.2

303.3 ± 22.4

(2.94 ± 0.13) × 109

(3.10 ± 1.41) × 109

Raw material-optimized RSM

802.0

803.3 ± 28.3

(7.94 ± 0.20) × 109

(8.05 ± 0.70) × 109

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