Rapid optimization of spore production from Bacillus amyloliquefaciens in submerged cultures based on dipicolinic acid fluorimetry assay

AMB Express

Table 4 Central composite design for soybean meal and corn meal associated with spore density by the fluorometric assay

Run	Models	Concentration (g/L)		Fluorescence intensity (AU)
Run	Models	Corn meal (x₅)	Soybean meal (x₆)	Experimental	Predicted
1	A0	11.4	10	626.5 ± 47.4	628.5
2	00	10	10	750.4 ± 55.4	763.2
3	+−	11	9	672.4 ± 38.4	688.7
4	0A	10	11.4	615.9 ± 26.2	625.2
5	00	10	10	765.6 ± 55.5	763.2
6	00	10	10	772.9 ± 59.9	763.2
7	−+	9	11	711.3 ± 54.1	717.4
8	0a	10	8.6	779.0 ± 58.6	746.7
9	a0	8.6	10	815.2 ± 55.6	790.2
10	−−	9	9	755.6 ± 52.7	790.8
11	++	11	11	601.2 ± 24.4	588.4

The experimental results are the means of two replicates of 1000-fold diluted spore samples. The symbols in the model column mean each factor at five different levels (−a, −, 0, +, A). The variables at a central coded value are considered at zero