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Table 4 Central composite design for soybean meal and corn meal associated with spore density by the fluorometric assay

From: Rapid optimization of spore production from Bacillus amyloliquefaciens in submerged cultures based on dipicolinic acid fluorimetry assay

Run Models Concentration (g/L) Fluorescence intensity (AU)
Corn meal (x5) Soybean meal (x6) Experimental Predicted
1 A0 11.4 10 626.5 ± 47.4 628.5
2 00 10 10 750.4 ± 55.4 763.2
3 +− 11 9 672.4 ± 38.4 688.7
4 0A 10 11.4 615.9 ± 26.2 625.2
5 00 10 10 765.6 ± 55.5 763.2
6 00 10 10 772.9 ± 59.9 763.2
7 −+ 9 11 711.3 ± 54.1 717.4
8 0a 10 8.6 779.0 ± 58.6 746.7
9 a0 8.6 10 815.2 ± 55.6 790.2
10 −− 9 9 755.6 ± 52.7 790.8
11 ++ 11 11 601.2 ± 24.4 588.4
  1. The experimental results are the means of two replicates of 1000-fold diluted spore samples. The symbols in the model column mean each factor at five different levels (−a, −, 0, +, A). The variables at a central coded value are considered at zero