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Table 1 Effects of metal ions with different concentrations on the spore yields of B. amyloliquefaciens BS-20 detected by the fluorometric assay

From: Rapid optimization of spore production from Bacillus amyloliquefaciens in submerged cultures based on dipicolinic acid fluorimetry assay

Concentration (mM)

Fluorescence intensity (AU)

Fe3+

Fe2+

Mn2+

Mg2+

Ca2+

Zn2+

0.0

996.7 ± 48.5

996.7 ± 48.5a

996.7 ± 48.5a

996.7 ± 48.5a

996.7 ± 48.5a

996.7 ± 48.5a

0.5

1080.0 ± 112.8a

1.0

1073.0 ± 26.5

1205.0 ± 108.9a

1661.5 ± 102.5b

1184.5 ± 71.4b

1067.0 ± 98.9ab

285.0 ± 18.4b

2.0

1019.0 ± 34.0

1527.0 ± 145.7b

1451.0 ± 75.0ab

1186.0 ± 19.8b

1526.5 ± 64.3c

226.0 ± 29.7bc

3.0

1104.0 ± 68.5

1624.5 ± 163.3b

1231.5 ± 79.9ab

1539.5 ± 77.1c

1451.0 ± 93.3c

175.0 ± 15.6c

4.0

1098.0 ± 55.5

1182.5 ± 120.9a

1097.5 ± 3.5bc

1219.0 ± 4.2b

65.0 ± 9.9d

5.0

1012.0 ± 29.5

1136.0 ± 90.5a

1068.5 ± 62.9bc

1203.5 ± 34.6b

35.0 ± 4.2d

  1. Mean values in the same column with different letters (a, b, c, d) are significantly different (P < 0.05). The final results are expressed as the mean ± standard deviation (n = 3) of 100-fold diluted spore samples