Skip to main content

Table 1 Effects of metal ions with different concentrations on the spore yields of B. amyloliquefaciens BS-20 detected by the fluorometric assay

From: Rapid optimization of spore production from Bacillus amyloliquefaciens in submerged cultures based on dipicolinic acid fluorimetry assay

Concentration (mM) Fluorescence intensity (AU)
Fe3+ Fe2+ Mn2+ Mg2+ Ca2+ Zn2+
0.0 996.7 ± 48.5 996.7 ± 48.5a 996.7 ± 48.5a 996.7 ± 48.5a 996.7 ± 48.5a 996.7 ± 48.5a
0.5 1080.0 ± 112.8a
1.0 1073.0 ± 26.5 1205.0 ± 108.9a 1661.5 ± 102.5b 1184.5 ± 71.4b 1067.0 ± 98.9ab 285.0 ± 18.4b
2.0 1019.0 ± 34.0 1527.0 ± 145.7b 1451.0 ± 75.0ab 1186.0 ± 19.8b 1526.5 ± 64.3c 226.0 ± 29.7bc
3.0 1104.0 ± 68.5 1624.5 ± 163.3b 1231.5 ± 79.9ab 1539.5 ± 77.1c 1451.0 ± 93.3c 175.0 ± 15.6c
4.0 1098.0 ± 55.5 1182.5 ± 120.9a 1097.5 ± 3.5bc 1219.0 ± 4.2b 65.0 ± 9.9d
5.0 1012.0 ± 29.5 1136.0 ± 90.5a 1068.5 ± 62.9bc 1203.5 ± 34.6b 35.0 ± 4.2d
  1. Mean values in the same column with different letters (a, b, c, d) are significantly different (P < 0.05). The final results are expressed as the mean ± standard deviation (n = 3) of 100-fold diluted spore samples