Fig. 2

Specific dissociation peak of WSSV positive samples by melt curve analysis with corresponding conventional PCR amplicon (148 bp) targeting VP28 gene of WSSV. The ethidium bromide-stained agarose gel (1.5%) was used to visualized 148 bp PCR amplicon generated by qVP28F and qVP28R primers. The Tm value (81.1 ± 17 °C) of real-time PCR specified the unified WSSV amplicon in the samples