Fig. 5

Time-course reactions for the biotransformations of PPT-type ginsenosides to APPT by DT-bgl combined with PF-bgl. a Ginsenoside R1. The reactions were performed at 80 °C in 50 mM citrate/phosphate buffer (pH 6.0) containing 1.0 mg ml⁻¹ R1, 4.0 mg ml⁻¹ DT-bgl, and 5 µg ml⁻¹ PF-bgl for 6 h. b PPT-type ginsenosides in notoginseng root extract. The reactions were performed at 80 °C in 50 mM citrate/phosphate buffer (pH 6.0) containing 1.0 mg ml⁻¹ total PPT-type ginsenosides, 5.0 mg ml⁻¹ DT-bgl, and 0.4 mg ml⁻¹ PF-bgl for 4 h. Data represent the means of three experiments, and error bars represent the standard deviation