Cost-effective downstream processing of recombinantly produced pexiganan peptide and its antimicrobial activity

AMB Express

Table 1 Mass balance of the downstream processing of DAMP4_var-pexiganan protein

Process	Protein yield (mg)^a	Protein recovery (%)
Cell lysis	32.9 ± 0.3	100
DNA removal	30.2 ± 0.2	91.8 ± 0.2
Protein-contaminant precipitation	28.2 ± 0.9	85.8 ± 1.9
DAMP4_var-pexiganan isolation^b	24.7 ± 0.5	75.0 ± 1.0
Buffer exchange	24.0 ± 0.6	72.9 ± 1.0

^aThe protein yield was measured by the area of the peak shown in RP-HPLC, except that the protein after cell lysis step was calculated based on SDS-PAGE gel using ImageJ software (Wibowo et al. 2017)
^bThe yield of DAMP4_var-pexiganan protein after isolation was measured after rinsing the protein precipitate against rinsing buffer (25 mM Tris–HCl, 1 M NaCl, pH 8, 2.2 M Na₂SO₄) and then solubilized in solubilizing buffer (25 mM Tris–HCl, 1 M NaCl, pH 8)