Skip to main content

Table 1 Mass balance of the downstream processing of DAMP4var-pexiganan protein

From: Cost-effective downstream processing of recombinantly produced pexiganan peptide and its antimicrobial activity

Process Protein yield (mg)a Protein recovery (%)
Cell lysis 32.9 ± 0.3 100
DNA removal 30.2 ± 0.2 91.8 ± 0.2
Protein-contaminant precipitation 28.2 ± 0.9 85.8 ± 1.9
DAMP4var-pexiganan isolationb 24.7 ± 0.5 75.0 ± 1.0
Buffer exchange 24.0 ± 0.6 72.9 ± 1.0
  1. aThe protein yield was measured by the area of the peak shown in RP-HPLC, except that the protein after cell lysis step was calculated based on SDS-PAGE gel using ImageJ software (Wibowo et al. 2017)
  2. bThe yield of DAMP4var-pexiganan protein after isolation was measured after rinsing the protein precipitate against rinsing buffer (25 mM Tris–HCl, 1 M NaCl, pH 8, 2.2 M Na2SO4) and then solubilized in solubilizing buffer (25 mM Tris–HCl, 1 M NaCl, pH 8)