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Table 5 Experimental design with actual and predicted responses for the Box–Behnken design where the independent variables were designated: X1—feather content, X2—concentration of MgSO4, X3—concentration of KH2PO4

From: New keratinolytic bacteria in valorization of chicken feather waste

Run X1 X2 X3 Actual response Predicted response
ln protein (μg/mL) Amino acid (mM) ln protein (μg/mL) Amino acid (mM)
1 − 1 − 1 0 5.453 45.29 5.389 37.86
2 + 1 − 1 0 6.483 57.81 6.407 74.60
3 − 1 + 1 0 4.934 47.69 5.010 30.90
4 + 1 + 1 0 5.926 166.51 5.990 173.94
5 − 1 0 − 1 5.198 43.83 5.124 68.52
6 + 1 0 − 1 6.521 155.99 6.458 156.47
7 − 1 0 + 1 5.414 48.75 5.477 48.27
8 + 1 0 + 1 6.065 164.78 6.140 140.09
9 0 − 1 − 1 6.070 112.83 6.209 95.56
10 0 + 1 − 1 5.879 165.05 5.878 157.14
11 0 − 1 + 1 6.292 84.72 6.294 92.63
12 0 + 1 + 1 5.966 106.17 5.827 123.43
13 0 0 0 5.793 157.72 5.727 148.93
14 0 0 0 5.677 167.31 5.727 148.93
15 0 0 0 5.771 133.74 5.727 148.93
16 0 0 0 5.666 136.94 5.727 148.93