New keratinolytic bacteria in valorization of chicken feather waste

AMB Express

Table 5 Experimental design with actual and predicted responses for the Box–Behnken design where the independent variables were designated: X₁—feather content, X₂—concentration of MgSO₄, X₃—concentration of KH₂PO₄

Run	X₁	X₂	X₃	Actual response		Predicted response
Run	X₁	X₂	X₃	ln protein (μg/mL)	Amino acid (mM)	ln protein (μg/mL)	Amino acid (mM)
1	− 1	− 1	0	5.453	45.29	5.389	37.86
2	+ 1	− 1	0	6.483	57.81	6.407	74.60
3	− 1	+ 1	0	4.934	47.69	5.010	30.90
4	+ 1	+ 1	0	5.926	166.51	5.990	173.94
5	− 1	0	− 1	5.198	43.83	5.124	68.52
6	+ 1	0	− 1	6.521	155.99	6.458	156.47
7	− 1	0	+ 1	5.414	48.75	5.477	48.27
8	+ 1	0	+ 1	6.065	164.78	6.140	140.09
9	0	− 1	− 1	6.070	112.83	6.209	95.56
10	0	+ 1	− 1	5.879	165.05	5.878	157.14
11	0	− 1	+ 1	6.292	84.72	6.294	92.63
12	0	+ 1	+ 1	5.966	106.17	5.827	123.43
13	0	0	0	5.793	157.72	5.727	148.93
14	0	0	0	5.677	167.31	5.727	148.93
15	0	0	0	5.771	133.74	5.727	148.93
16	0	0	0	5.666	136.94	5.727	148.93