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Table 1 Plasmids used in this study

From: Plasmid to generate Mycobacteria mutants

Plasmids

Application

Source

pMind

Used to construct pMDX and as template to amplify hygromycin resistance gene

Robertson, Brian D (Blokpoel et al. 2005)

pJRD215

Used as template to amplify streptomycin resistance gene

Professor Xiangmei Liu (Chen et al. 2011)

pMY769

Used as template to amplify gap fragments

Professor Francesca Forti (Forti et al. 2009)

pGOAL19

Used as template to amplify the promotor pAG85

Tanya Parish (Parish and Stoker 2000)

pEGFP-C1

Used as template to amplify EGFP gene

Doc Zhang (Zhang et al. 1996)

pJV53

Used to facilitate recombination

van Kessel, J.C. (van Kessel and Hatfull 2007)

pMDX

The plasmid tool

This study