AMB Express

Table 1 Effects of ions and chemical reagents on agarase activity

From: Extracellular expression of a novel β-agarase from Microbulbifer sp. Q7, isolated from the gut of sea cucumber

Ions and chemical reagents	Concentrations	Relative activity (%)
Na⁺	5 mM	113.34 ± 0.57
	20 mM	97.93 ± 1.02
	50 mM	96.94 ± 0.75
	5 mM	116.46 ± 0.43
Fe²⁺	20 mM	82.75 ± 0.73
Fe²⁺	50 mM	77.65 ± 0.82
Cu²⁺	5 mM	6.2 ± 0.45
K⁺	5 mM	97.26 ± 0.63
Ca²⁺	5 mM	96.78 ± 0.34
Zn²⁺	5 mM	14.97 ± 1.02
Mg²⁺	5 mM	76.92 ± 0.36
Li⁺	5 mM	92.00 ± 0.58
Fe³⁺	5 mM	34.65 ± 1.09
EDTA	5 mM	86.47 ± 1.44
SDS	5 mM	99.26 ± 0.78
DTT	5 mM	103.44 ± 0.99
Urea	5 mM	96.01 ± 0.73
Tween-80	0.5% (v/v)	89.72 ± 0.82
Triton-100	0.5% (v/v)	100.94 ± 0.69
β-Mercaptoethanol	0.5% (v/v)	141.05 ± 0.74

The data shown are representative of three independent experiments
The activity measured under the standard condition is defined as 100%
The concentration indicates the final reagents concentrations in the reaction

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