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Table 1 Effects of ions and chemical reagents on agarase activity

From: Extracellular expression of a novel β-agarase from Microbulbifer sp. Q7, isolated from the gut of sea cucumber

Ions and chemical reagents Concentrations Relative activity (%)
Na+ 5 mM 113.34 ± 0.57
20 mM 97.93 ± 1.02
50 mM 96.94 ± 0.75
5 mM 116.46 ± 0.43
Fe2+ 20 mM 82.75 ± 0.73
50 mM 77.65 ± 0.82
Cu2+ 5 mM 6.2 ± 0.45
K+ 5 mM 97.26 ± 0.63
Ca2+ 5 mM 96.78 ± 0.34
Zn2+ 5 mM 14.97 ± 1.02
Mg2+ 5 mM 76.92 ± 0.36
Li+ 5 mM 92.00 ± 0.58
Fe3+ 5 mM 34.65 ± 1.09
EDTA 5 mM 86.47 ± 1.44
SDS 5 mM 99.26 ± 0.78
DTT 5 mM 103.44 ± 0.99
Urea 5 mM 96.01 ± 0.73
Tween-80 0.5% (v/v) 89.72 ± 0.82
Triton-100 0.5% (v/v) 100.94 ± 0.69
β-Mercaptoethanol 0.5% (v/v) 141.05 ± 0.74
  1. The data shown are representative of three independent experiments
  2. The activity measured under the standard condition is defined as 100%
  3. The concentration indicates the final reagents concentrations in the reaction