Transglycosylation of gallic acid by using Leuconostoc glucansucrase and its characterization as a functional cosmetic agent

AMB Express

Table 1 Central composite design matrix for the experimental design and predicted responses for gallic acid glucoside synthesis

Run no.	Coded levels			Gallic acid glucoside synthesis (mM)
Run no.	X₁	X₂	X₃	Actual	Predicted
1	150	300	150	29.0	13/9
2	560	300	150	22.4	10.9
3	150	1000	150	86.4	68.0
4	560	1000	150	80.9	69.0
5	150	300	500	43.5	24.5
6	560	300	500	32.5	19.9
7	150	1000	500	82.9	63.4
8	560	1000	500	78.7	62.8
9	10.2	650	325	21.6	49.5
10	699.8	650	325	30.7	46.5
11	355	61.4	325	0.2	19.8
12	355	1238.6	325	77.4	101.4
13	355	650	30.7	6.2	25.0
14	355	650	619.3	3.9	28.7
15	355	650	325	122.7	103.9
16	355	650	325	92.9	103.9
17	355	650	325	104.6	103.9
18	355	650	325	98.2	103.9
19	355	650	325	114.8	103.9
20	355	650	325	98.2	103.9

Y = − 158.9 + 0.32X₁ + 0.24X₂ + 0.63X₃ − 0.00047X ²₁ − 0.00013X ²₂ − 0.00089X ²₃ + 0.0000139X₁X₂ − 0.000011X₁X₃ − 0.000062X₂X₃