Inhibitory effect of streptococci on the growth of M. catarrhalis strains and the diversity of putative bacteriocin-like gene loci in the genomes of S. pneumoniae and its relatives

AMB Express

Table 1 Characterization of study strains

Strain ID (VKM^a ID)	Species	Provider^b/isolation year	Isolate source/patient age	Identification
Strain ID (VKM^a ID)	Species	Provider^b/isolation year	Isolate source/patient age	OPT test (CO₂ atm.)	Bile solubility	Latex agglutination test (“Slidex® pneumo-kit”)	Serotype^d	Sequence type (MLST)
Spn_357 (VKM B-3128)	S. pneumoniae	EMSUMD/2008	Cerebrospinal fluid/adu	pos.	pos.	pos.	23F	ST 81
Spn_2009 (VKM B-3127)	S. pneumoniae	EMSUMD/2008	Blood/n.d.	pos.	pos.	pos.	22 F/A	ST 1470
Spn-NT_13856 (VKM B-3125)	S. pneumoniae	SCCH/2013	Nasopharynx/ped	neg.	neg.	neg.	NT^b	ST 2996
Spn-NT_2298 (VKM B-3126)	S. pneumoniae	SCCH/2013	Nasopharynx/ped	pos.	neg.	neg.	NT	ST 2996
Spspn_G42 (VKM B-3123)	S. pseudopneumoniae	SCCH/2013	Nasopharynx/ped	neg.^c	neg.	neg.	–	–
Spspn_22725 (VKM B-3124)	S. pseudopneumoniae	SCCH/2013	Nasopharynx/ped	neg.^c	neg.	neg.	–	–
Sm_11/5 (VKM B-3130)	S. mitis	NACPP/2009	Nasopharynx/adu	neg.	neg.	neg.	–	–
Sm_13/39 (VKM B-3131)	S. mitis	NACPP/2009	Nasopharynx/ped	neg.	neg.	neg.	–	–
Sm_18/56 (VKM B-3129)	S. mitis	NACPP/2009	Nasopharynx/adu	neg.	neg.	neg.	–	–

pos. positive, neg. negative, n.d. no data, adu adult, ped pediatric, NT non-typeable
^aVKM is all-Russian collection of microorganisms (http://www.vkm.ru/) in which all strains under study are deposited
^bEMSUMD—A.I. Evdokimov Moscow State University of Medicine and Dentistry, Moscow, Russia; SCCH—Federal State Budgetary Inst. “Scientific Center of Children Health” of RAMS, Moscow, Russia; NACPP—National Agency for Clinical Pharmacology and Pharmacy, Moscow, Russia
^cSome zones of inhibition (less than 14 mm) near OPT discs were observed for these strains under culturing in CO₂ atmosphere, in contrast to culturing in air, where zones of inhibition were 18–20 mm and more
^dSerotypes of pneumococcal strains were determined by inspection of the nucleotide sequences of genes coding the fragments of capsules, in accordance with the CDC recommendation (http://www.cdc.gov/streplab/protocols.html) (see “Materials and methods”)