Strategies for improving production performance of probiotic Pediococcus acidilactici viable cell by overcoming lactic acid inhibition

AMB Express

Table 4 Effect of agitation speed on growth of P. acidilactici in batch fermentation using 2 L stirred tank bioreactor

Kinetic parameter	Agitation speed (rpm)
Kinetic parameter	200	300	400
Maximum viable cell concentration (cfu/mL)	1.2 × 10¹² ± 0.17^b	1.8 × 10¹² ± 0.12^a	1.0 × 10¹² ± 0.23^c
Time to reach maximum viable cell concentration (h)	12^a	12^a	12^a
Viable cell yield (cfu/g_Glucose)	1.4 × 10¹⁴ ± 0.18^b	2.0 × 10¹⁴ ± 0.1^a	1.2 × 10¹⁴ ± 0.17^c
Viable cell productivity (cfu/mL h)	9.9 × 10¹⁰ ± 0.16^b	1.5 × 10¹¹ ± 0.15^a	8.3 × 10¹⁰ ± 0.12^c
Maximum lactic acid concentration (g/L)	12.71 ± 0.16^b	13.21 ± 0.14^a	11.77 ± 0.23^c
Lactic acid yield (g/g_Glucose)	0.91 ± 0.07^a	0.92 ± 0.08^a	0.91 ± 0.12^a
Lactic acid productivity (g/L h)	0.53 ± 0.08^a	0.54 ± 0.08^a	0.48 ± 0.1^b

The results presented are the average of triplicate experiments and are expressed as mean ± standard deviation
^a,b,cMean values in the same row with different superscripts are significantly different from each other (P < 0.05)