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AMB Express
Fig. 1 | AMB Express

Fig. 1

From: Production of poly-γ-glutamic acid by a thermotolerant glutamate-independent strain and comparative analysis of the glutamate dependent difference

Fig. 1

a The colonial morphology of strain GXG-5 on agar plate. The plate medium composed of glucose 25.0 g/L, soy peptone 3.0 g/L, NH4NO3 17.0 g/L, NaCl 5.0 g/L, K2HPO4 2.5 g/L, and MgSO4·7H2O 1.0 g/L. Initial pH was adjusted to 7.0 ± 0.1. b The microscopic features of GXG-5 (scanning electromicroscope, ×10,000). c Gram staining of GXG-5 (optical microscope, Olympus CX41, ×100). d Neighbor-joining phylogenetic tree based on 16S rDNA gene sequences showing the position of strain GXG-5 (GenBank ID: KY711183) among its closely related organisms. Numbers in parentheses are NCBI accession numbers of published sequences. Bootstrap values (1000 replicates) are mentioned at the nodes. The scale bar represents 0.01 nucleotide substitution per position

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